Sub-Millisecond Single Molecule Fluorescence Imaging Combined with Dual Optical Tweezers on DNA Tethers
Sub-Millisecond Single Molecule Fluorescence Imaging Combined with Dual Optical Tweezers on DNA Tethers
复制标题
DNA 系绳上的亚毫秒单分子荧光成像与双光镊相结合
DOI：
10.1016/j.bpj.2011.11.979
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发表时间：
2012
影响因子：
3.4
通讯作者：
Llorente Garcia I
中科院分区：
文献类型：
--
作者：
Llorente Garcia I
我们提出了一种新的光学设计，它同时结合了多色荧光显微镜与单分子灵敏度和亚毫秒时间分辨率，以及精确的定位控制和诊断的光镊。这种多功能的成像设置，目前正在开发中，将用于一些DNA系链实验，在这些实验中，DNA将被固定在两个激光器之间，捕获的珠粒以及系链和各种结合的蛋白质复合物将同时显现。
We present a novel optical design which simultaneously combines multi-color fluorescence microscopy with single molecule sensitivity and sub-millisecond temporal resolution, together with the accurate positioning control and diagnosis of optical tweezers.This versatile imaging set-up, currently under development, will serve a number of DNA tether experiments in which the DNA will be held between two laser-trapped beads and both the tether and a variety of bound protein complexes will be visualised simultaneously.