3,3′,4,4′,5-Pentachlorobiphenyl influences mitochondrial apoptosis pathway in granulosa cells
3,3′,4,4′,5-Pentachlorobiphenyl influences mitochondrial apoptosis pathway in granulosa cells
复制标题
3,3-,4,4-,5-五氯联苯影响颗粒细胞线粒体凋亡途径
DOI：
10.1002/jcb.28801
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发表时间：
2019-09-01
影响因子：
4
通讯作者：
Liu，Shuzhen
中科院分区：
文献类型：
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作者：
Zhong，Tao;Zhang，Jianmei;Liu，Shuzhen
3，3 ′，4，4 ′，5-多氯联苯（PCB 126）是一种持久性有机环境污染物，可影响生物体的免疫、神经功能和生殖等多种生物活性。在我们的研究中，我们的目的是研究PCB 126对颗粒细胞（GCs）的影响。培养24小时后，从PMSG处理小鼠的卵巢收集GC。然后将GC与10 pg/mL、100 pg/mL和10 ng/mL的PCB 126再孵育24小时。在这些步骤之后，收集暴露的GC用于进一步实验。我们的数据表明，GC的数量在10 ng/mL的PCB 126减少。10 ng/mLPCB 126组细胞核固缩，染色质浓缩，凋亡细胞明显增多。此外，PCB 126处理后凋亡执行蛋白caspase-3的表达增加。与线粒体凋亡途径相关的Bax、Bcl-2和Bim的表达也受到不同程度的影响。提示PCB 126影响GCs凋亡，线粒体凋亡途径参与了PCB 126诱导GCs凋亡的过程。
3,3′,4,4′,5‐Polychlorinated biphenyl (PCB126) is a persistent organic environmental pollutant which can affect various biological activities of organisms, such as immunity, neurological function, and reproduction. In our study, we aimed to investigate the effects of PCB126 on granulosa cells (GCs). GCs were collected from ovaries in PMSG‐treated mice, after 24 hours culture. GCs were then incubated with 10 pg/mL, 100 pg/mL, and 10 ng/mL of PCB126 for another 24 hours. Following these steps, exposed GCs were collected for further experimentation. Our data showed that the number of GCs in the 10 ng/mL PCB126 decreased. Meanwhile, pyknotic nuclei and condensed chromatin increased, while the apoptotic cells in the 10 ng/mL PCB126 group were significantly increased. Furthermore, the expression of the apoptotic executive protein caspase‐3 increased after PCB126 treatment. The expression of Bax, Bcl‐2, and Bim related to the mitochondrial apoptosis pathway were also influenced to different degrees. Thus, our data suggested that PCB126 affect the GCs apoptosis, and mitochondrial apoptosis pathway was involved in this process.