Steric and Electrostatic Complementarity in the Assembly of Two-Dimensional Virus Arrays
Steric and Electrostatic Complementarity in the Assembly of Two-Dimensional Virus Arrays
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DOI：
10.1021/la903114s
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发表时间：
2010-03-02
期刊：
影响因子：
3.9
通讯作者：
DeYoreo, James J.
中科院分区：
文献类型：
--
作者：
Cheung, Chin Li;Rubinstein, Alexander I.;DeYoreo, James J.
生物分子的高度有序组装为研究纳米尺度物体的组织原理提供了有力的手段。利用渗透压消耗效应和滴干法，将二维豇豆花叶病毒阵列以有序方式组装在云母上。通过调节病毒溶液中聚乙二醇表面活性剂的浓度，系统地控制病毒阵列的堆积，从菱形堆积到六边形堆积。发现病毒阵列的方向和堆积对称性可以通过样品溶液中表面活性剂的浓度来调节。提出了本系统的唯象模型来解释表面活性剂影响下的组装阵列形态。发现相邻病毒衣壳的空间和静电互补是控制包装对称性的关键因素。
A highly ordered assembly of biological molecules provides a powerful means to study the orgnizational principles of objects at the nanoscale. Two-dimensional cowpea mosaic virus arrays were assembled in an ordered manner on mica using osmotic depletion effects and a drop-and-dry method. The packing of the virus array was controlled systematically from rhombic packing to hexagonal packing by modulating the concentrations of poly(ethylene glycol) surfactant in the virus solutions. The orientation and packing symmetry of the virus arrays were found to be tuned by the concentrations of surfactants in the sample solutions. A phenomenological model fo the present system is proposed to explain the assembly array morphology under the influence of the surfactant. Steric and electrostatic complementary of neighboring virus capsids is found to be the key factors in controlling the symmetry of packing.