Gentamicin coating of metallic implants reduces implant-related osteomyelitis in rats
Gentamicin coating of metallic implants reduces implant-related osteomyelitis in rats
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DOI：
10.1016/s8756-3282(03)00050-4
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发表时间：
2003-05-01
期刊：
影响因子：
4.1
通讯作者：
Raschke, M
中科院分区：
文献类型：
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作者：
Lucke, M;Schmidmaier, G;Raschke, M
抗生素预防是骨科手术的常规程序。各种局部抗生素输送技术用于减少骨和软组织相关感染。本研究的目的是评估新型可生物降解、负载庆大霉素的聚（D,L-丙交酯）（PDLLA）矫形器械涂层在预防植入物相关骨髓炎方面的功效。 30 只 Sprague Dawley 大鼠的胫骨髓腔被金黄色葡萄球菌（10(3) 个菌落形成单位）污染。同时植入未涂层（II 组）、涂有 PDLLA（III 组）或涂有 PDLLA + 10% 庆大霉素（IV 组）的钛克氏针。接受磷酸盐缓冲盐水和未涂覆的克氏针的 10 只动物作为对照组（第 1 组）。随访时间为 6 周。每周进行一次胫骨 X 光检查，进行血细胞计数，并测定体温和体重。处死后通过组织学和微生物学分析评估感染。第 11 组和第 III 组的所有动物均出现微生物学、组织学和放射学感染迹象，包括骨质破坏和软组织肿胀。对照组的所有动物均保持无菌。与H组和111组的培养物相比，IV组植入物的培养物显示细菌生长显着减少，并且IV组的三个植入物保持无菌。与第二组和第三组相比，庆大霉素包被组的进一步放射学和组织学感染迹象显着减少。所有组之间的体重、体温和血液参数均未观察到显着差异。在该动物模型中，局部应用含有 PDLLA 和 10% 庆大霉素的抗生素涂层骨科器械可显着减少与植入物相关的感染。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
Antibiotic prophylaxis is a routine procedure in orthopedic surgery. Various local antibiotic delivery techniques are used to reduce bone-and soft tissue-related infection. The objective of this study was to evaluate the efficacy of a new biodegradable, gentamicin-loaded poly(D,L-lactide) (PDLLA) coating of orthopedic devices in preventing implant-related osteomyelitis. The medullary cavities of tibiae in 30 Sprague Dawley rats were contaminated with Staphylococcus aureus (10(3) colony forming units). Simultaneously titanium Kirschner wires, uncoated (group II), coated with PDLLA (group III), or coated with PDLLA + 10% gentamicin (group IV), were implanted. Ten animals that received phosphate-buffered saline and uncoated Kirschner wires served as controls (group 1). Follow-up was 6 weeks. In weekly intervals X-rays of the tibiae were performed, blood counts were taken, and body temperature and weight were determined. After sacrifice infection was evaluated by histological and microbiological analysis. All animals of groups 11 and III developed microbiological, histological, and radiological signs of infection, including osseous destruction and soft tissue swelling. All animals of the control group remained sterile. Cultures of implants of group IV showed significantly reduced bacterial growth compared to cultures of groups H and 111, and three implants of group IV remained sterile. Further radiological and histological signs of infection were significantly reduced in the gentamicin-coated group compared to groups II and III. No significant differences in body weight, body temperature, and blood parameters between all groups were observed. Local application of antibiotic-coated orthopedic devices containing PDLLA and 10% gentamicin significantly reduced implant-related infection in this animal model. (C) 2003 Elsevier Science (USA). All rights reserved.