Analysis of the survival of mature human eosinophils: interleukin-5 prevents apoptosis in mature human eosinophils.
Analysis of the survival of mature human eosinophils: interleukin-5 prevents apoptosis in mature human eosinophils.
复制标题
成熟人嗜酸性粒细胞存活率分析：白细胞介素5可防止成熟人嗜酸性粒细胞凋亡。
作者：
Yuji Yamaguchi;Toshio Suda;Shigeo Ohta;K. Tominaga;Yasusada Miura;Tadashi Kasahara
我们和其他小组之前已经证明，白细胞介素 5 (IL-5) 在体外液体培养系统中维持成熟嗜酸性粒细胞的活力。成熟的嗜酸性粒细胞不增殖，但在 IL-5 存在的情况下仍能维持其存活。使用该培养系统，我们研究了 IL-5 介导的生存机制。在不存在人IL-5 (hIL-5)的情况下，成熟的嗜酸性粒细胞在4天后死亡，而在存在hIL-5的情况下，它们存活长达10天。当在琼脂凝胶电泳上分析培养的嗜酸性粒细胞的DNA提取物时，在不存在hIL-5的情况下观察到明显的DNA片段化，而在含有hIL-5的培养物48小时中未观察到显着的DNA片段化。 DNA 片段化早在 hIL-5 剥夺后 6 至 12 小时就出现了。与此同时，IL-5 刺激成熟嗜酸性粒细胞中总 RNA 和蛋白质的合成，但不诱导 DNA 合成。由于放线菌酮或放线菌素 D 阻碍了 hIL-5 对细胞凋亡的保护，因此这种现象似乎需要一些新的 RNA 和蛋白质合成。这些发现表明，IL-5 通过诱导新的 RNA 和蛋白质合成来维持成熟嗜酸性粒细胞的存活，从而抑制细胞凋亡。
We and other groups have previously shown that interleukin-5 (IL-5) maintained the viability of mature eosinophils in an in vitro liquid culture system. Mature eosinophils did not proliferate but their survival was maintained in the presence of IL-5. Using this culture system, we investigated the mechanism of IL-5-mediated survival. In the absence of human IL-5 (hIL-5) mature eosinophils succumbed after 4 days, while in the presence of hIL-5 they survived up to 10 days. When DNA extracts of cultured eosinophils were analyzed on an agar gel electrophoresis, marked DNA fragmentation was observed in the absence of hIL-5, while no significant DNA fragmentation was observed in the culture with hIL-5 for 48 hours. The DNA fragmentation appeared as early as 6 to 12 hours after hIL-5 deprivation. Concomitantly, IL-5 stimulated total RNA and protein synthesis, but did not induce DNA synthesis in mature eosinophils. Because cycloheximide or actinomycin D impeded the protection of apoptosis by hIL-5, some new RNA and protein synthesis appeared to be required in this phenomena. These findings indicate that IL-5 maintains survival of mature eosinophils with induction of new RNA and protein synthesis, thus leading to the inhibition of apoptosis.
纯化的集落刺激因子可增强人中性粒细胞和嗜酸性粒细胞的体外存活：一种快速、灵敏的集落刺激因子微量测定法。
影响因子：
20.3
作者：
Begley,CG;Lopez,AF;Nicola,NA;Warren,DJ;Vadas,MA;Sanderson,CJ;Metcalf,D
通讯作者：
Metcalf,D
影响因子：
56.9
作者：
KOURY, MJ;BONDURANT, MC
通讯作者：
BONDURANT, MC