Cre activity causes widespread apoptosis and lethal anemia during embryonic development
Cre activity causes widespread apoptosis and lethal anemia during embryonic development
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DOI：
10.1002/dvg.20353
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发表时间：
2007-12-01
期刊：
影响因子：
1.5
通讯作者：
Papaioannou, Virginia E.
中科院分区：
文献类型：
--
作者：
Naiche, L. A.;Papaioannou, Virginia E.
Cre介导的靶向loxP位点的切除被广泛用于以时间或组织特异性方式删除或激活基因表达。我们研究了三个先前描述的Cre等位基因，发现Cre活性单独导致显着的发育缺陷，如造血活性的丧失和显着上调的细胞凋亡在许多胚胎组织中的两个这些线。这些结果表明cre表达产生的假表型可以混淆遗传学分析。我们还发现，最近发表的研究未能包括Cre阳性对照，因此可能将作用归因于靶基因，这实际上部分或全部归因于Cre毒性。这一信息将是至关重要的，在评估先前发表的工作，利用cre等位基因和设计未来的实验。
Cre-mediated excision of targeted loxP sites is widely used to delete or to activate gene expression in temporal or tissue-specific fashions. We examine three previously described cre alleles and find that Cre activity alone causes dramatic developmental defects, such as loss of hematopoietic activity and dramatically upregulated apoptosis in many embryonic tissues in two of these lines. These results demonstrate that cre expression generates spurious phenotypes that can confound genetics analyses. We also find that most recently published studies fail to include cre-positive controls, and thus may have attributed roles to a targeted gene, which were in reality partly or wholly due to Cre toxicity. This information will be critical in both evaluating previously published work using cre alleles and in designing future experiments.