The presence of specific binding sites on boar spermatozoa for porcine relaxin and its action on their motility characteristics
The presence of specific binding sites on boar spermatozoa for porcine relaxin and its action on their motility characteristics
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公猪精子上猪松弛素特异性结合位点的存在及其对其运动特性的作用
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
O. Sherwood
中科院分区：
文献类型：
--
作者：
T. Kohsaka;H. Sasada;H. Takahara;E. Sato;K. Bamba;O. Sherwood
本研究的目的是研究猪精子是否具有松弛素的特异性结合位点，并研究松弛素对精子运动特性的影响。从猪附睾尾部收集精子。对于松弛素结合研究，将洗涤后的附睾尾部精子涂抹在载玻片上，并通过依次应用生物素化松弛素探针、与1 nm胶体金缀合的抗生物素免疫球蛋白G和用于信号放大的银来鉴定结合松弛素的特异性位点。在运动特性研究中，附睾尾部精子在改良KRB + 2%附睾尾部液中稀释300倍（约1 × 107个/ml）。在CO2培养箱中于37 ℃预孵育30分钟后，将精子等分试样置于覆盖有矿物油的培养皿中，并与猪松弛素混合。松弛素和精子的浓度分别为100 ng/ml和5 × 106个细胞/ml。将等分试样在相同条件下孵育一定的规定时间。孵育30、60、90和120 min后，使用录像机记录精子活力。作为运动特征的评价，检查了运动百分比、向前进展等级和速度。松弛素结合研究的结果表明，松弛素特异性结合的中段和尾部。此外，在顶体帽和头部颈部可见结合。研究松弛素对公猪精子运动特性的影响的运动研究结果表明，松弛素并没有引起运动百分比的显著改变，但它改善了向前的进展。松弛素治疗90分钟产生了显着的1.4倍的精子速度相比，对照组的增强。这些结果表明，松弛素激活精子运动通过一个特定的受体。
The purpose of this investigation was to examine whether boar spermatozoa possess specific binding sites for relaxin and to examine what effects relaxin actually exerts on their motility characteristics. Boar spermatozoa were collected from cauda epididymides. For the relaxin binding study, washed cauda epididymal spermatozoa were smeared on glass slides, and specific sites that bind relaxin were identified by sequential application of a biotinylated relaxin probe, antibiotine immunoglobulin G conjugated to 1 nm colloidal gold, and silver for signal amplification. For the motility characteristics study, cauda epididymal spermatozoa were diluted 300-fold in modified KRB + 2% cauda epididymal fluid (approximately 1 × 10 7 cell/ml). After preincubation for 30 min at 37 C in a CO2 incubator, the sperm aliquots were placed in culture dishes covered with mineral oil and mixed with porcine relaxin. The concentrations of relaxin and spermatozoa in the aliquot were 100 ng/ml and 5 × 106 cells/ml, respectively. The aliquots were incubated for a certain defined period under the same conditions. After 30, 60, 90 and 120 min of incubation, sperm motility was recorded using a videotape recorder. As an evaluation of motility characteristics, the percent motility, grade of forward progression and velocity were examined. The results of the relaxin binding study demonstrated that relaxin bound with specificity to the midpiece and tail. In addition, bindings were visible at the acrosome cap and neck of the head. The results of the motility study which examined the effects of relaxin on the motility characteristics of boar sperm showed that relaxin did not cause a significant alteration in percent motility, but it improved forward progression. Relaxin treatment for 90 min produced a significant 1.4-fold enhancement in sperm velocity compared to that of control. These results suggest that relaxin activates sperm motility through a specific receptor.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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DOI：
10.1177/37.8.2666509
发表时间：
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期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
Sokol,RZ;Wang,XS;Lechago,J;Johnston,PD;Swerdloff,RS
通讯作者：
Swerdloff,RS
影响因子：
6.7
作者：
M. Essig;C. Schoenfeld;R. Amelar;L. Dubin;G. Weiss
通讯作者：
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