Functional cytochrome oxidase histochemistry in the honeybee brain
Functional cytochrome oxidase histochemistry in the honeybee brain
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DOI：
10.1016/s0006-8993(00)02016-3
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发表时间：
2000-03-24
期刊：
影响因子：
2.9
通讯作者：
Gauthier, M
中科院分区：
文献类型：
--
作者：
Armengaud, C;Causse, N;Gauthier, M
用细胞色素氧化酶（CO）组织化学方法研究了蜜蜂脑内神经代谢在手术和化学处理后的变化。通过触角输入剥夺，α叶中的CO染色显著减少。化学刺激（50 mM K+）与触角叶（AL）中CO染色的增加和萼（Cal）基底环的减少有关。烟碱配体吡虫啉（10（-4）M）的应用导致增加CO标记在30分钟内在所有的结构分析。用较低浓度（10（-8）M）处理导致Cal和中心体（CB）染色减少。我们的结论是，CO组织化学可用于识别蜜蜂脑中的胆碱能配体的靶结构。(C)2000 Elsevier Science B. V.保留所有权利。
The variations of neural metabolism induced by surgical and chemical treatments were studied in the honeybee brain by the means of cytochrome oxidase (CO) histochemistry. CO staining is considerably reduced in the alpha-lobe by antennal input deprivation. Chemical stimulation (50 mM K+) was linked to an increase of CO staining in antennal lobes (AL) and to a decrease in the basal ring of calyces (Cal). Application of the nicotinic ligand imidacloprid (10(-4) M) resulted in increased CO labelling within 30 min in all the structures analysed. Treatment with a lower concentration (10(-8) M) resulted in reduced staining in Cal and central body (CB). We conclude that CO histochemistry can be used to identify the target structures of cholinergic ligands in the honeybee brain. (C) 2000 Elsevier Science B.V. All rights reserved.