5α-Cholest-8(14)-en-3β-ol, a Possible Intermediate in the Biosynthesis of Cholesterol ENZYMATIC CONVERSION TO CHOLESTEROL AND ISOLATION FROM RAT SKIN
5α-Cholest-8(14)-en-3β-ol, a Possible Intermediate in the Biosynthesis of Cholesterol ENZYMATIC CONVERSION TO CHOLESTEROL AND ISOLATION FROM RAT SKIN
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5α-Cholest-8(14)-en-3β-ol，胆固醇生物合成中可能的中间体酶促转化为胆固醇并从大鼠皮肤中分离
DOI：
10.1016/s0021-9258(18)63585-9
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发表时间：
1969
影响因子：
4.8
通讯作者：
G. Schroepfer
中科院分区：
文献类型：
--
作者：
Wen;B. Lutsky;G. Schroepfer
用化学合成法制备了[3α-3H]-5α-胆甾-8（14）-烯-3 β-醇。已证明该化合物的标记物可通过完整大鼠掺入肝脏胆固醇中。已确定5α-胆甾-8（14）-烯-3 β-醇可被大鼠肝匀浆转化为5α-胆甾-7-烯-3 β-醇和胆固醇。在厌氧条件下，将[3α-3H]-5α-胆甾-8（14）-烯-3 β-醇与大鼠肝匀浆制备物孵育后，添加的底物回收率保持不变。已证明大鼠皮肤中存在具有5α-胆甾-8（14）-烯-3 β-醇预期特性的甾醇。
[3α-3H]-5α-Cholest-8(14)-en-3β-ol has been prepared by chemical synthesis. The incorporation of the label of this compound into liver cholesterol by intact rats has been demonstrated. The convertibility of 5α-cholest-8(14)-en-3β-ol to 5α-cholest-7-en-3β-ol and cholesterol by rat liver homogenates has been established. Upon incubation of [3α-3H]-5α-cholest-8(14)-en-3β-ol with a rat liver homogenate preparation under anaerobic conditions, the added substrate was recovered unchanged. The presence of a sterol with the expected properties of 5α-cholest-8(14)-en-3β-ol in rat skin has been demonstrated.