Immunohistochemical analysis of protein expression during Aedes aegypti development.
Immunohistochemical analysis of protein expression during Aedes aegypti development.
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DOI：
10.1101/pdb.prot5510
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发表时间：
2010-10-01
影响因子：
--
通讯作者：
Duman-Scheel, Molly
中科院分区：
文献类型：
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作者：
Clemons, Anthony;Flannery, Ellen;Duman-Scheel, Molly
吸血蚊子，包括登革热和黄热病媒介埃及伊蚊，传播世界上许多最致命的疾病。这类疾病在发展中国家死灰复燃，并对发达国家的流行病爆发构成明显威胁。最近的蚊子基因组计划激发了人们对通过对媒介昆虫进行遗传操作来控制节肢动物传播疾病的潜力的兴趣，而调节发育的基因产物尤其令人感兴趣。蛋白质表达的免疫组织化学分析的该方案可用于分析Ae中感兴趣的发育蛋白质的表达。埃及人胚胎、幼虫和蛹，这对于开发特定发育组织的标记物至关重要。
Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and gene products that regulate development are of particular interest. This protocol for immunohistochemical analysis of protein expression can be used to analyze expression of developmental proteins of interest in Ae. aegypti embryos, larvae, and pupae, which will be critical for the development of markers for particular developing tissues.