Coating of polymeric surfaces for immunoassay by a forced adsorption technique.
Coating of polymeric surfaces for immunoassay by a forced adsorption technique.
复制标题
通过强制吸附技术进行免疫测定的聚合物表面涂层。
DOI：
10.1080/15321818308057007
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
R. Anacker
中科院分区：
文献类型：
--
作者：
K. Hechemy;R. Anacker
描述了一种用于涂覆聚合物表面的强制吸附技术。该技术简单，并且允许我们使用（i）相对不纯的配体涂覆在胶乳表面上和（ii）用于制备大体积批次胶乳试剂的最小量的配体。与被动吸附试剂的变异性不同，试剂批次间的血清学结果具有高度重现性。这种技术的使用可以扩展到固相免疫测定的平面涂层。
A forced adsorption technique for coating polymeric surfaces is described. This technique is simple and allows us to use (i) relatively impure ligands to be coated on latex surface and (ii) the minimum amount of ligands for preparation of large-volume lots of latex reagent. The serologic results are highly reproducible from lot to lot of the reagent, in contrast to the variability found with passively adsorbed reagents. Use of this technique could be extended to coat flat surfaces for solid-phase immunoassays.