Nucleotides in 16S rRNA that are required in unmodified form for features recognized by ribosomal protein S8.
Nucleotides in 16S rRNA that are required in unmodified form for features recognized by ribosomal protein S8.
复制标题
16S rRNA 中的未修饰形式的核苷酸是核糖体蛋白 S8 识别特征所必需的。
DOI：
--
复制
发表时间：
1983
影响因子：
14.9
通讯作者：
B. Ehresmann
中科院分区：
文献类型：
--
作者：
D. L. Thurlow;C. Ehresmann;B. Ehresmann
采用损伤选择实验方法鉴定了16S rRNA中未修饰的核苷酸，这些核苷酸是大肠杆菌核糖体蛋白S8特异性识别所必需的。在与S8形成络合物之前，用焦碳酸二乙酯或25%联氨在完全变性条件下处理16S rRNA。将RNA结合片段与非结合片段分离后，用苯胺处理S8相关片段，分析其修饰碱基的含量。在这些片段中发现的始终未被修饰的核苷酸位于稳定螺旋的基部附近（包括碱基581-656）或靠近3'近端螺旋的顶端。发现一个较小的S8核糖核蛋白颗粒含有沿3'方向延伸到671位的片段。
Nucleotides in 16S rRNA which are required in unmodified form for specific recognition of ribosomal protein S8 from Escherichia coli were identified using a damage-selection experimental approach. Prior to complex formation with S8, 16S rRNA was treated under fully denaturing conditions with either diethyl pyrocarbonate or 25% hydrazine. Following separation of bound from unbound fragments of RNA, those associated with S8 were analyzed for their content of modified bases by treatment with aniline. Nucleotides found to be consistently unmodified in such fragments were located near the base of a stable helix (encompassing bases 581-656) or near the apex of the helix on the 3' proximal side. A minor S8 ribonucleoprotein particle was found to contain fragments which extended in the 3' direction to position 671.