Dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (Sparus aurata) juveniles
Dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (Sparus aurata) juveniles
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DOI：
10.1016/j.cbpa.2015.09.012
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发表时间：
2016-01-01
影响因子：
2.3
通讯作者：
Peres, H.
中科院分区：
文献类型：
--
作者：
Coutinho, F.;Castro, C.;Peres, H.
本试验旨在评价饲粮中添加谷氨酰胺对鳙鱼生产性能、肠道营养物质吸收能力、肝脏和肠道谷氨酰胺代谢及氧化状态的影响。分别饲喂4种等脂质（18%脂质）和等氮质（43%蛋白质）饲料，分别添加0、0.5、1和2%谷氨酰胺，为期6周。饲料中谷氨酰胺水平对鱼的生产性能、体组成和肠道营养吸收能力没有影响。肝脏和肠道谷氨酰胺酶（GINase）、谷氨酰胺合成酶（GSase）、丙氨酸转氨酶、天冬氨酸转氨酶和谷氨酸脱氢酶活性也不受饲粮中添加谷氨酰胺的影响。肠道中谷氨酰胺酶活性比肝脏高，GSase/GINase比肝脏低2倍，这表明肠道对谷氨酰胺的能量产生比肝脏高。肝脏过氧化氢酶和葡萄糖-6-磷酸脱氢酶活性较高，而肠道谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性及氧化谷胱甘肽含量较高，这似乎揭示了肠道抗氧化反应对谷胱甘肽的依赖性较高。饲粮中添加谷氨酰胺可提高肝脏和肠道中总谷胱甘肽和还原性谷胱甘肽含量以及肠道超氧化物歧化酶活性，但对脂质过氧化值没有影响。总之，我们确定了肝脏和肠道谷氨酰胺代谢和抗氧化反应之间的差异，并阐明了饲料中添加谷氨酰胺对鳙鱼抗氧化反应的潜在影响。(c) 2015 Elsevier Inc.版权所有。
A study was undertaken to evaluate dietary glutamine supplementation effects on gilthead sea bream performance, intestinal nutrient absorption capacity, hepatic and intestinal glutamine metabolism and oxidative status. For that purpose gilthead sea bream juveniles (mean weight 13.0 g) were fed four isolipidic (18% lipid) and isonitrogenous (43% protein) diets supplemented with 0, 0.5, 1 and 2% glutamine for 6 weeks. Fish performance, body composition and intestinal nutrient absorption capacity were not affected by dietary glutamine levels. Hepatic and intestinal glutaminase (GINase), glutamine synthetase (GSase), alanine aminotransferase, aspartate aminotransferase and glutamate dehydrogenase activities were also unaffected by dietary glutamine supplementation. In the intestine GINase activity was higher and GSase/GINase ratio was two-fold lower than in the liver, suggesting a higher use of glutamine for energy production by the intestine than by the liver. The liver showed higher catalase and glucose-6-phosphate dehydrogenase activities, while the intestine presented higher glutathione peroxidase and glutathione reductase activities and oxidised glutathione content, which seems to reveal a higher glutathione dependency of the intestinal antioxidant response. Total and reduced glutathione contents in liver and intestine and superoxide dismutase activity in the intestine were enhanced by dietary glutamine, though lipid peroxidation values were not affected. Overall, differences between liver and intestine glutamine metabolism and antioxidant response were identified and the potential of dietary glutamine supplementation to gilthead sea bream's antioxidant response was elucidated. (c) 2015 Elsevier Inc. All rights reserved.