A rapid, high-yield method of producing mono-[125I]A14 iodoinsulin.
A rapid, high-yield method of producing mono-[125I]A14 iodoinsulin.
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一种快速、高产率生产单-[125I]A14 碘胰岛素的方法。
DOI：
10.1080/10826068408070637
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
B. Ginsberg
中科院分区：
文献类型：
--
作者：
M. Lioubin;M. Meier;B. Ginsberg
We have developed a rapid method for producing homogeneous mono-[125I]A14 iodoinsulin with high specific activity and yield. After iodination by the lactoperoxidase method, the labeled peptides were applied to a C18 Porasil pre-column, washed with aqueous buffer to eliminate the free [125I]-iodide and placed "in-line" with a C-18 HPLC column; mono-[125I]A14 iodoinsulin was then eluted isocratically with 29% acetonitrile in 16 minutes. The labeled hormone was extremely stable, and proved suitable for various biological studies.
