Schistosoma mansoni:: Chemoreception through n-acetyl-D-galactosamine-containing receptors in females offers insight into increased severity of schistosomiasis in individuals with blood group A
Schistosoma mansoni:: Chemoreception through n-acetyl-D-galactosamine-containing receptors in females offers insight into increased severity of schistosomiasis in individuals with blood group A
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DOI：
10.1016/j.exppara.2007.12.016
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发表时间：
2008-05-01
影响因子：
2.1
通讯作者：
Eveland, L. K.
中科院分区：
文献类型：
--
作者：
Haseeb, M. A.;Thors, C.;Eveland, L. K.
体外研究了曼氏血吸虫雌虫体表暴露的碳水化合物在化学感受中的作用。用花生凝集素（PNA）、大豆凝集素（SBA）和小麦胚芽凝集素（WGA）处理性成熟的雌性，并在生物测定系统中测定它们对雄性排泄分泌（ES）产物的迁移反应。未处理和凝集素处理的雌性的随机分布是相似的（p = >0.05）。PNA处理的女性无反应的1.0小时后，他们表现出轻微的排斥ES产品。SBA处理的雌性动物在3.0 h内对ES产品无反应。WGA处理的女性无反应的1.5小时后，他们表现出轻微的吸引力男性ES产品。尝试通过使用特异性糖凝集素结合抑制剂来恢复化学吸引力;化学吸引力在N-乙酰基-D-半乳糖胺（GaINAe）处理的雌性中恢复得最好。这些数据表明，含GaINAc的受体，可能是GP 50，主要的SBA结合的雌激素受体，介导雌激素受体的化学感受。这些发现可以解释观察到的不同血型个体之间发病率的差异。具有血型B或0的个体可以提供部分保护，因为它们的天然抗A或通过建立针对血型A红细胞共有的含GaINAc结构的更有效的抗体应答。(C)2008年爱思唯尔公司All rights reserved.
The role of exposed surface carbohydrates in chemoreception in Schistosoma mansoni females was investigated in vitro. Sexually mature females were treated with peanut (PNA), soybean (SBA) or wheat germ (WGA) agglutinin, and their migration in response to excretory-secretory (ES) products of males was measured in a bioassay system. Random distribution of untreated and lectin-treated females was similar (p = >0.05). PNA-treated females were unresponsive for 1.0 h, after which they exhibited slight repulsion to ES products. SBA-treated females did not respond to ES products for 3.0 h. WGA-treated females were unresponsive for 1.5 h, after which they exhibited slight attraction to male ES products. Attempts were made to restore chemoattraction by using specific sugar inhibitors of lectin-binding; chemoattraction was best restored in n-acetyl-D-galactosamine (GaINAe)-treated females. These data suggest that GaINAc-containing receptors, possibly GP50, the major SBA-binding schistosome glycan, mediate chemoreception in schistosome females. These findings may explain the observed differences in morbidity between individuals with different blood groups. Individuals with blood group B or 0 may afford partial protection because of their natural anti-A or by mounting a more efficient antibody response directed against GaINAc-containing structures shared by blood group A red blood cells. (C) 2008 Elsevier Inc. All rights reserved.