COLD-STORAGE OF PERIPHERAL-NERVES - AN IN-VITRO ASSAY OF CELL VIABILITY AND FUNCTION
COLD-STORAGE OF PERIPHERAL-NERVES - AN IN-VITRO ASSAY OF CELL VIABILITY AND FUNCTION
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DOI：
10.1002/glia.440100206
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发表时间：
1994-02-01
期刊：
影响因子：
6.2
通讯作者：
BUNGE, RP
中科院分区：
文献类型：
--
作者：
LEVI, ADO;EVANS, PJ;BUNGE, RP
神经库作为修复周围神经损伤大缺损的供体材料来源的发展，需要了解冷藏对这些潜在神经移植物细胞活力和功能的影响。将人类和大鼠的周围神经片段保存在威斯康星大学冷库溶液（UW）中，温度为4摄氏度，保存时间< 12小时，3天，1、2或3周。细胞活力最初是通过储存神经的外植体在培养中的细胞生长程度来评估的，然后通过分离培养的神经外植体并计算每毫克周围神经的细胞类型和数量来进一步量化。从储存的（对照和1周和2周）神经中获得大鼠雪旺细胞（SCs），在培养中检测其髓鞘化背根神经节（DRG）神经元的功能能力。我们的研究结果表明，人类和大鼠周围神经在冷藏3周后含有少量的活SCs和成纤维细胞，而在冷藏1周后，人类周围神经内的活细胞数量明显减少。尽管它们的数量减少，但一些储存2周的大鼠神经的SCs能够在培养中形成髓鞘。这些结果表明，短时间（< 1周）的冷库将导致潜在的周围神经移植物含有大量的功能细胞，而长期（大于或等于3周）的冷库周围神经将含有很少的活细胞。(C) 1994 Wiley-Liss, Inc。
The development of a nerve bank as a source of donor material to repair large defects in peripheral nerve injuries requires an understanding of the influence of cold storage on cell viability and function in these potential nerve grafts. Segments of peripheral nerves from both human and rat were stored in University of Wisconsin Cold Storage Solution (UW) at 4 degrees C for < 12 h, 3 days, and 1, 2, or 3 weeks. Cellular viability was initially assessed by the degree of cellular outgrowth from explants of the stored nerves placed in culture, and then further quantitated by dissociating the cultured nerve explants and calculating the type and number of cells per milligram of peripheral nerve. Rat Schwann cells (SCs) obtained from the stored (control and 1 and 2 weeks) nerves were tested for their functional ability to myelinate dorsal root ganglion (DRG) neurons in culture. Our findings indicate that human and rat peripheral nerves contain few viable SCs and fibroblasts after 3 weeks of cold storage with the quantity of viable cells within the human cold stored peripheral nerves decreasing significantly after 1 week of cold storage. Despite their reduced number, some SCs from rat nerves stored up to 2 weeks are capable of myelinating DRG axons in culture. These results suggest that short intervals (< 1 week) of cold storage will result in potential peripheral nerve grafts containing large populations of functional cells, while long-term (greater than or equal to 3 weeks) cold stored peripheral nerves will contain few viable cells. (C) 1994 Wiley-Liss, Inc.