Metabolomics Study of Human Urinary Metabolome Modifications After Intake of Almond (Prunus dulcis (Mill.) DA Webb) Skin Polyphenols
Metabolomics Study of Human Urinary Metabolome Modifications After Intake of Almond (Prunus dulcis (Mill.) DA Webb) Skin Polyphenols
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DOI：
10.1021/pr100639v
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发表时间：
2010-11-01
影响因子：
4.4
通讯作者：
Andres-Lacueva, Cristina
中科院分区：
文献类型：
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作者：
Llorach, Rafael;Garrido, Ignacio;Andres-Lacueva, Cristina
杏仁作为坚果家族的一部分，是生物化合物的重要来源，特别是杏仁皮被认为是多酚的重要来源，包括黄烷-3-醇和黄酮醇。多酚代谢可能会产生几类代谢物，这些代谢物往往比它们的饮食前体更具生物学活性，也可能成为杏仁多酚摄入量的一个强有力的新生物标志物。为了研究单次服用杏仁皮提取物后24小时内尿液代谢组的变化，24名志愿者(n=24)在研究前和研究期间分别服用了无多酚饮食48小时的杏仁皮酚类化合物(n=12)或安慰剂(n=12)。分别于服药前(-2~0h)、服药后(0-2 h、2-6 h、6-10 h、10-24 h)采集尿样，采用液-质联用法(LC-Q-TOF)和多元统计分析(主成分分析和潜在结构正交投影)进行分析。相关生物标志物的推定鉴定显示，与单剂杏仁提取物有关的代谢物共有34种，包括宿主，特别是微生物区系。代谢物。据我们所知，这是首次通过代谢组学方法在摄入黄烷-3-醇后的人体样本中鉴定出羟基苯丙酸、羟苯丙酸和羟苯基乙酸的偶联物。结果表明，这种非靶向的方法可以提供新的摄取生物标志物，有助于食物代谢组作为人类尿代谢组的重要组成部分的发展。
Almond, as a part of the nut family, is an important source of biological compounds, and specifically, almond skins have been considered an important source of polyphenols, including flavan-3-ols and flavonols. Polyphenol metabolism may produce several classes of metabolites that could often be more biologically active than their dietary precursor and could also become a robust new biomarker of almond polyphenol intake. In order to study urinary metabolome modifications during the 24 h after a single dose of almond skin extract, 24 volunteers (n = 24), who followed a polyphenol-free diet for 48 h before and during the study, ingested a dietary supplement of almond skin phenolic compounds (n = 12) or a placebo (n = 12). Urine samples were collected before ((-2)-0 h) and after (0-2 h, 2-6 h, 6-10 h, and 10-24 h) the intake and were analyzed by liquid chromatography-mass spectrometry (LC-q-TOF) and multivariate statistical analysis (principal component analysis (PCA) and orthogonal projection to latent structures (OPLS)). Putative identification of relevant biomarkers revealed a total of 34 metabolites associated with the single dose of almond extract, including host and, in particular, microbiota. metabolites. As far as we know, this is the first time that conjugates of hydroxyphenylvaleric, hydroxyphenylpropionic, and hydroxyphenylacetic acids have been identified in human samples after the consumption of flavan-3-ols through a metabolomic approach. The results showed that this non-targeted approach could provide new intake biomarkers, contributing to the development of the food metabolome as an important part of the human urinary metabolome.