ACTIVATION OF A NOVEL HUMAN TRANSFORMING GENE, RET, BY DNA REARRANGEMENT
ACTIVATION OF A NOVEL HUMAN TRANSFORMING GENE, RET, BY DNA REARRANGEMENT
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DOI：
10.1016/0092-8674(85)90115-1
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
COOPER, GM
中科院分区：
文献类型：
--
作者：
TAKAHASHI, M;RITZ, J;COOPER, GM
用人淋巴瘤DNA转染NIH 3T3细胞，检测到一种新的转化基因。肿瘤DNA在初级转染中诱导单一焦点，而转化的NIH细胞的DNA在二级和三级检测中诱导转化效率很高。从三级转化DNA中分离出约37 kb的人类序列分子克隆。印迹杂交表明，转化基因由两个片段组成，这两个片段在正常人和原发性淋巴瘤dna中都不连锁。人类DNA的两个片段在转化的NIH细胞中共转录，但在任何被检查的人类细胞中都没有。因此，转化基因似乎是通过两个不相连的人类DNA片段之间的重组激活的，可能是在转染过程中通过协整激活的。
A novel transforming gene was detected by transfection of NIH 3T3 cells with human lymphoma DNA. The tumor DNA induced a single focus in primary transfections, whereas DNAs of transformed NIH cells induced transformation with high efficiencies in secondary and tertiary assays. Molecular clones spanning about 37 kb of human sequence were isolated from tertiary transformant DNA. Blot hybridization indicated that the transforming gene consisted of two segments that were unlinked in both normal human and primary lymphoma DNAs. The two segments of human DNA were cotranscribed in transformed NIH cells but not in any human cells examined. The transforming gene thus appeared to be activated by recombination between two unlinked human DNA segments, possibly by cointegration during transfection.