Branched‐chain α‐keto acid dehydrogenase kinase content in rat skeletal muscle is decreased by endurance training
Branched‐chain α‐keto acid dehydrogenase kinase content in rat skeletal muscle is decreased by endurance training
复制标题
DOI：
10.1080/15216549800202302
复制
发表时间：
1998-05
期刊：
影响因子：
4.6
通讯作者：
H. Fujii;Y. Shimomura;T. Murakami;N. Nakai;Tasuku Sato;Masashige Suzuki;R. Harris
中科院分区：
文献类型：
--
作者：
H. Fujii;Y. Shimomura;T. Murakami;N. Nakai;Tasuku Sato;Masashige Suzuki;R. Harris
跑步运动使训练和未训练大鼠骨骼肌中支链α-酮酸脱氢酶复合物的活性状态升高，但前者的升高程度显著高于后者。为了阐明运动诱导的复合物活化的训练效果的机制，通过蛋白质印迹分析测量肌肉中负责通过磷酸化使复合物失活的支链α-酮酸脱氢酶激酶的蛋白质量。耐力训练降低了30%的肌肉中的激酶蛋白的含量，这表明这种减少是参与的机制，更大的激活复杂的训练大鼠的运动。
The activity state of branched‐chain α‐keto acid dehydrogenase complex in skeletal muscle was elevated by running exercise in trained and untrained rats, but level of this elevation was significantly greater in the former than in the latter. To elucidate the mechanism of the training effect on the exercise‐induced activation of the complex, a protein amount of branched‐chain α‐keto acid dehydrogenase kinase, which is responsible for inactivation of the complex by phosphorylation, in the muscle was measured by the Western blot analysis. Endurance training decreased the content of the kinase protein in the muscle by ∼30%, suggesting that this decrease is involved in the mechanisms for greater activation of the complex by exercise in trained rats.