AMPLIFICATION AND SEQUENCING OF THE CONTROL REGIONS OF BK AND JC VIRUS FROM HUMAN URINE BY POLYMERASE CHAIN-REACTION
AMPLIFICATION AND SEQUENCING OF THE CONTROL REGIONS OF BK AND JC VIRUS FROM HUMAN URINE BY POLYMERASE CHAIN-REACTION
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DOI：
10.1016/0042-6822(91)90069-n
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发表时间：
1991-02-01
期刊：
影响因子：
3.7
通讯作者：
SUBRAMANI, S
中科院分区：
文献类型：
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作者：
FLAEGSTAD, T;SUNDSFJORD, A;SUBRAMANI, S
BKV和JCV的不同毒株在DNA复制起点附近的非编码区表现出显著的异质性。因此，对自然发生的变异进行表征，作为了解这种高度变异性的起源和生物学意义的第一步，是非常有意义的。本文报道了用聚合酶链式反应从艾滋病患者、骨髓移植受者和其他患者组的尿液中扩增和测序BKV和JCVDNA的控制区。我们的结果支持这样的结论，即BK(WW)及其变异体构成了迄今为止在测试人群中最流行的毒株。从挪威的一些患者中分离到一种新的菌株，命名为BK(TU)。含有BK(WW)的尿液在细胞培养中繁殖后产生BK(TU)，而BK(TU)在同一过程中不改变其控制区的序列。JCV分离株与Y.Yogo、T.Kitamura、C.Sugimoto、T.Ueki、Y.Aso、K.Hara和F.Taguchi(J.Virol.，1990，，3139-3143)报道的几个从尿液中克隆的菌株几乎相同。这个原型毒株可能代表了在人类群体中循环的JCV，JCV分离株的各种调控序列可能通过缺失和扩增进化而来。
The various strains of BKV and JCV exhibit a remarkable degree of heterogeneity in the noncoding region near the origin of DNA replication. It is of great interest, therefore, to characterize the naturally occurring variants as a first step towards the attainment of an understanding of the origin and the biological significance of this hypervariability. In this paper we report the use of polymerase chain reaction for amplification and sequencing of the control regions of BKV and JCV DNAs from urines of AIDS patients, bone marrow transplantation recipients, and other patient groups. Our results support the conclusion that BK(WW) and its variants constitute the most prevalent strain in the human population tested so far. A new strain, designated BK(TU), was isolated from some patients from Norway. Urine inocula containing BK(WW) gave BK(TU) after propagation in cell culture, while BK(TU) did not change the sequence of its control region during the same procedure. The JCV isolates were almost identical with several strains molecularly cloned from the urine reported by Y. Yogo, T. Kitamura, C. Sugimoto, T. Ueki, Y. Aso, K. Hara, and F. Taguchi (J. Virol., 1990, 64, 3139–3143). This archetypal strain may represent the JCV circulating in the human population, from which various regulatory sequences of JCV isolates could have evolved by deletions and amplifications.