Isotope-coded chemical reporter and acid-cleavable affinity reagents for monitoring protein sulfenic acids
Isotope-coded chemical reporter and acid-cleavable affinity reagents for monitoring protein sulfenic acids
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DOI：
10.1016/j.bmcl.2011.04.115
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Carroll, Kate S.
中科院分区：
文献类型：
--
作者：
Truong, Thu H.;Garcia, Francisco J.;Carroll, Kate S.
We have developed an approach that allows relative quantification of protein sulfenic acids using a pair of light and heavy isotope labled probes, DAz-2 and d(6)-DAz-2. In conjunction with a new complementary acid-cleavable linker, Yn-ACL, we demonstrate that tagged peptides are successfully labeled, enriched, and fully characterized by LC-MS/MS analysis. Overall, this method can be applied to map sites of cysteine oxidation and compare protein sulfenylation in normal and disease states. Published by Elsevier Ltd.
