PROJECTIONS OF PHYSIOLOGICALLY CHARACTERIZED GLOBULAR BUSHY CELL AXONS FROM THE COCHLEAR NUCLEUS OF THE CAT
PROJECTIONS OF PHYSIOLOGICALLY CHARACTERIZED GLOBULAR BUSHY CELL AXONS FROM THE COCHLEAR NUCLEUS OF THE CAT
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DOI：
10.1002/cne.903040305
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发表时间：
1991-02-15
影响因子：
2.5
通讯作者：
YIN, TCT
中科院分区：
文献类型：
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作者：
SMITH, PH;JORIS, PX;YIN, TCT
我们用HRP填充的玻璃微电极从猫的梯形体中的30个单独的球状丛状细胞轴突进行轴突内记录。结果发现，特征频率（CFs）高于3 kHz的细胞，其短声刺激周时间直方图（PSTH）在低声强时为类初级，在高声强时为类初级并伴有切迹（PL（N））或起始时伴有低持续活动（O（L））。CF在1和3 kHz之间的细胞表现出相同的响应特征，在响应的持续区域中的尖峰锁相到刺激音。与具有可比CF的第八神经纤维相比，具有低于1 kHz的CF的细胞显示具有异常高水平的同步性的锁相PSTH。当CFs为1-3 kHz的细胞以低于1 kHz的音调呈现时，也注意到这种特殊的锁相。虽然在对侧脑干中，从起源的索马到终末野的球状浓密细胞轴突没有完全填充，但在人群中区分出一些一致的解剖特征。除了一个外，所有有髓轴突都在中间穿过中线，梯形体的大纤维成分。在同侧，轴突通常由1 ~ 4个侧支发出，其主要靶点为后橄榄周核（PPO）和斜方体外侧核（LNTB）。同侧副支的次要终止部位为背外侧橄榄周围核（DLPO）和外侧上级橄榄（LSO）。在斜方体内侧核（MNTB）内，轴突发出1 ~ 2个Held神经盏。其他三个主要的侧支来自对侧轴突，并支配一组一致的地区。其中一个朝向尾部，以支配面神经核腹内侧的区域。另一组沿第6神经背侧行，支配橄榄周围核（DMPO）。第三个侧支朝向外侧丘系（VNLL）的腹侧核，偶尔发出小的侧支。虽然每一个注射的轴突引起了一个侧支支配的MNTB，它不一定引起所有其他三个侧支。
We made intraaxonal recordings from 30 individual globular bushy cell axons in the trapezoid body of the cat using HRP-filled glass microelectrodes. With subsequent HRP injection, we determined their axonal projection patterns.For cells with characteristic frequencies (CFs) above 3 kHz, short-tone peristimulus time histograms (PSTHs) at CF were typically primarylike at low tone intensities and primarylike with notch (PL(N)) or onset with low sustained activity (O(L)) at higher stimulus levels. Cells with CFs between 1 and 3 kHz showed the same response features with the spikes in the sustained region of the response phase-locked to the stimulus tone. Cells with CFs below 1 kHz showed phase-locked PSTHs with exceptionally high levels of synchrony compared to eighth nerve fibers with comparable CFs. This exceptional phase-locking was also noted when cells with CFs of 1-3 kHz were presented with tones below 1 kHz.Although the globular bushy cell axons were not completely filled from the soma of origin to terminal fields in the contralateral brainstem, a number of consistent anatomical features were distinguished in the population. All but one of the myelinated axons crossed the midline in the middle, large fiber component of the trapezoid body. Ipsilaterally, the axon always gave off from one to four collateral branches whose major targets were the posterior periolivary nucleus (PPO) and the lateral nucleus of the trapezoid body (LNTB). Minor termination sites for ipsilateral collateral branches were the dorsolateral periolivary nucleus (DLPO) and the lateral superior olive (LSO). Contralaterally the axon gave rise to one or two calyces of Held in the medial nucleus of the trapezoid body (MNTB). Three other major collateral branches arose from the contralateral axon and innervated a consistent set of areas. One headed caudally to innervate an area just ventromedial to the facial nucleus. Another followed the sixth nerve dorsally to innervate the dorsomedial periolivary nucleus (DMPO). A third collateral headed rostrally toward the ventral nucleus of the lateral lemniscus (VNLL), giving off occasional small sidebranches. Although each injected axon gave rise to a collateral that innervated the MNTB, it did not necessarily give rise to all three of the other collateral branches.