EVIDENCE FOR AN ABNORMAL PROFILE OF INTERLEUKIN-4 (IL-4), IL-5, AND GAMMA-INTERFERON (GAMMA-IFN) IN PERIPHERAL-BLOOD T-CELLS FROM PATIENTS WITH ALLERGIC EOSINOPHILIC GASTROENTERITIS
EVIDENCE FOR AN ABNORMAL PROFILE OF INTERLEUKIN-4 (IL-4), IL-5, AND GAMMA-INTERFERON (GAMMA-IFN) IN PERIPHERAL-BLOOD T-CELLS FROM PATIENTS WITH ALLERGIC EOSINOPHILIC GASTROENTERITIS
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DOI：
10.1007/bf01540983
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发表时间：
1994-09-01
影响因子：
9.1
通讯作者：
METCALFE, DD
中科院分区：
文献类型：
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作者：
JAFFE, JS;JAMES, SP;METCALFE, DD
过敏性嗜酸性胃肠炎的特征是总IgE、食物抗原特异性IgE升高，组织和血液嗜酸性粒细胞增多。由于淋巴因子IL-4、IL-5和γ-干扰素在体外调节IgE合成和嗜酸性粒细胞生成，我们研究了在过敏性嗜酸性粒细胞性胃肠炎中它们的产生是否存在不平衡。为了探讨这一假设，三个成人过敏性嗜酸性胃肠炎患者进行了研究。对这些患者外周血单核细胞的流式细胞术研究未发现T细胞活化或T细胞数量或亚群紊乱的证据。T细胞能够在体外正常的促有丝分裂活化。IL-4和IL-5的产生与促有丝分裂刺激显著升高。大多数IL-4和IL-5的产生是通过CD 4（+）T细胞。3例患者的CD 4（+）T淋巴细胞和2例患者的CD 8（+）T淋巴细胞在无丝裂原的情况下合成IL-5。丝裂原刺激的GM-CSF和γ-干扰素由CD 4（+）T细胞合成正常。通过逆转录/聚合酶链反应检测来自内窥镜活检的总细胞RNA中的趋化因子mRNA。来自对照受试者的粘液活检和来自过敏性嗜酸性胃肠炎患者的大多数活检含有低于10（-8）μ g IL-5 mRNA/1 μ g总细胞mRNA。在过敏性嗜酸性胃肠炎患者的活检组织中，通过逆转录/聚合酶链反应未检测到γ-干扰素mRNA，但在对照组中存在。这些淋巴因子异常与过敏性嗜酸性粒细胞性胃肠炎中IgE升高和嗜酸性粒细胞增多一致，表明靶向T淋巴细胞的策略可能有效治疗这种疾病。
Allergic eosinophilic gastroenteritis is characterized by elevated total IgE, specific IgE to food antigens, and eosinophilia of tissue and blood. Because the lymphokines IL-4, IL-5, and gamma-interferon, regulate IgE synthesis, and eosinophilopoiesis in vitro, we examined whether there is an imbalance in their production in allergic eosinophilic gastroenteritis. To explore this hypothesis, three adult patients with allergic eosinophilic gastroenteritis were studied. Flow cytometric studies of peripheral blood mononuclear cells from these patients did not reveal evidence of T cell activation or disturbance of T cell numbers or subsets. T cells were capable of normal mitogenic activation in vitro. IL-4 and IL-5 production were markedly elevated with mitogenic stimulation. Most IL-4 and IL-5 production was by CD4(+) T cells. Synthesis of IL-5 by CD4(+) T lymphocytes in three patients and CD8(+) T lymphocytes in two patients occurred in the absence of mitogen. Mitogen-stimulated GM-CSF and gamma-interferon synthesis by CD4(+) T cells was normal. Lymphokine mRNA in total cellular RNA derived from endoscopic biopsies was examined by reverse transcription/polymerase chain reaction. Mucosal biopsies from control subjects and most biopsies from allergic eosinophilic gastroenteritis patients contained less than 10(-8) mu g IL-5 mRNA/1 mu g total cellular mRNA. gamma-Interferon mRNA was not detected by reverse transcription/polymerase chain reaction in biopsies from patients with allergic eosinophilic gastroenteritis but was present in controls. These lymphokine abnormalities are consistent with the elevated IgE and eosinophilia seen in allergic eosinophilic gastroenteritis and suggest that strategies targeting T lymphocytes may be efficacious in treatment of this disease.