Four classes of intercellular channels between glial cells in the CNS
Four classes of intercellular channels between glial cells in the CNS
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DOI：
10.1523/jneurosci.3303-03.2004
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发表时间：
2004-05-05
影响因子：
5.3
通讯作者：
Paul, DL
中科院分区：
文献类型：
--
作者：
Altevogt, BM;Paul, DL
星形胶质细胞与其他星形胶质细胞和少突胶质细胞形成广泛的缝隙连接。中枢神经系统胶质细胞之间的连接通讯可能是至关重要的，因为差距连接通道形成蛋白，连接蛋白Cx32和Cx47的丢失，导致严重的脱髓鞘。然而，中枢神经系统神经胶质细胞表达至少六个连接蛋白，这些蛋白质的细胞起源和关系尚未确定。我们制作了一个Cx29报告小鼠，其中连接蛋白编码序列被替换为组织学标记，用于证明Cx29，Cx32和Cx47在少突胶质细胞中特异性表达。为了确定星形胶质细胞和少突胶质细胞连接蛋白之间的关系，我们使用半薄切片的双重和三重免疫荧光显微镜（
Astrocytes form extensive gap junctions with other astrocytes and with oligodendrocytes. Junctional communication between CNS glia is likely of critical importance because loss of the gap junction channel-forming proteins, connexins Cx32 and Cx47, result in severe demyelination. However, CNS glia express at least six connexins, and the cellular origins and relationships of these proteins have not been determined. We produced a Cx29 reporter mouse in which the connexin coding sequence was replaced with a histological marker, which was used to demonstrate that Cx29, Cx32, and Cx47 are expressed specifically in oligodendrocytes. To determine the relationships between astrocyte and oligodendrocyte connexins, we used double- and triple-immunofluorescence microscopy using semithin sections (