In situ chemistry of osteoporosis revealed by synchrotron infrared microspectroscopy
In situ chemistry of osteoporosis revealed by synchrotron infrared microspectroscopy
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DOI：
10.1016/s8756-3282(03)00233-3
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发表时间：
2003-10-01
期刊：
影响因子：
4.1
通讯作者：
Chance, MR
中科院分区：
文献类型：
--
作者：
Huang, RY;Miller, LM;Chance, MR
骨密度降低是骨质疏松症的一个众所周知的特征，但对骨的化学成分变化或这种化学变化对骨折风险的影响知之甚少。使用卵巢切除的食蟹猴（食蟹猴）作为绝经期骨质疏松症发病的模型，我们研究了骨的微观化学变化的同步辐射红外显微光谱测量作为卵巢切除术后的时间的函数。结果表明，卵巢切除术后1年或2年形成的皮质骨，通过荧光染料标记鉴定，与假手术对照组相比，具有较高的磷酸盐含量（PO（4）（3-/）基质比），较低的碳酸盐含量（CO 32-/基质比）和更多的成熟胶原交联（不可还原交联/可还原交联比）。卵巢切除术后的小梁骨显示磷酸盐含量无变化，碳酸盐含量较低，未成熟胶原交联。用骨转换抑制剂（癸酸诺龙）治疗可将大部分卵巢切除术诱导的皮质骨化学变化逆转至卵巢完整对照组的水平，但对骨小梁几乎没有影响。这些结果表明，卵巢切除术后新合成的骨在特定的骨区域内与健康骨在化学上不同，这可能有助于骨质疏松症中骨质量的降低。(C)2003年爱思唯尔公司All rights reserved.
Reduced bone density is a well-known feature of osteoporosis, yet little is known about the changes in the chemical composition of bone or the impact of such chemical changes on fracture risks. Using ovariectomized cynomolgus monkeys (Macaca fascicularis) as a model for the menopausal onset of osteoporosis, we examined the microscopic chemical changes of bone measured by synchrotron infrared microspectroscopy as a function of time after ovariectomy. The results demonstrate that cortical bone formed I or 2 years after ovariectomy, as identified by fluorochrome labeling, has a higher phosphate content (PO(4)(3-/)matrix ratio), a lower carbonate content (CO32-/matrix ratio), and more mature collagen cross-links (nonreducible cross-link/reducible cross-link ratio) than that formed in sham-operated controls. Trabecular bone after ovariectomy shows no changes in phosphate content, a lower carbonate content, and immature collagen cross-linking. Treatment with a bone turnover suppressor, (nandrolone decanoate) reverses most of the ovariectomy-induced chemical changes in the cortical bone to the levels of the ovary-intact controls, but has little effect on the trabecular bone. These results demonstrate that bone newly synthesized after ovariectomy is chemically different from healthy bone within specific bone regions, which may contribute to reduced bone quality in osteoporosis. (C) 2003 Elsevier Inc. All rights reserved.