The latest hype on Hyp-O-glycosylation codes
The latest hype on Hyp-O-glycosylation codes
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DOI：
10.1016/s0031-9422(01)00029-2
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发表时间：
2001-06-01
期刊：
影响因子：
3.8
通讯作者：
Kieliszewski, MJ
中科院分区：
文献类型：
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作者：
Kieliszewski, MJ
当代糖生物学反映了对糖蛋白及其生物学作用的强烈兴趣。通过Nor O-糖基化添加Nor O是精确的而不是随机的，并且形成独特的相互作用分子表面。我们将这些保存良好的糖基化物命名为糖调节剂，以强调其功能意义。因此，阐明决定糖添加的糖基化密码是一个重要的目标。这里的重点是细胞壁蛋白的Hyp O-糖基化。这涉及两个连续的翻译后修饰，脯氨酸羟基化和糖基化。肽序列而不是构象似乎决定了这些修饰。Hyp糖基化以两种不同的模式发生：Hyp阿拉伯糖基化和Hyp半乳糖基化。Hyp邻接假设预测邻接Hyp残基的阿拉伯糖基化和成簇的非邻接Hyp的半乳糖基化。Hyp糖基化密码子的解析涉及推定的糖基化肽作为简单重复肽的设计和表达。因此，重复的（Ser-Hyp），定向Hyp半乳糖基化导致阿拉伯半乳聚糖多糖排他地添加到所有非连续Hyp残基，和一个新的AGP。另一种来自阿拉伯胶糖蛋白的重复肽，含有连续和非连续Hyp，指导Hyp糖基化的两种模式。此外，（Ser-Hyp）系列的表达证实了连续Hyp的阿拉伯糖基化。因此。Hyp邻接假说是功能基因组学工具箱中的有用的预测工具。(C)2001爱思唯尔科技有限公司版权所有。
Contemporary glycobiology reflects the intense interest in glycoproteins and their biological roles. Addition of saccharides by Nor O-glycosylation is precise rather than random and forms a uniquely interactive molecular surface. We designate these well con served glycomotifs as glycomodules to emphasize their functional significance. Thus, elucidation of the glycosylation codes that determine saccharide addition is a significant goal. The focus here is on the Hyp O-glycosylation of cell wall proteins. This involves two consecutive posttranslational modifications, proline hydroxylation and glycosylation. Peptide sequence rather than conformation seems to determine these modifications. Hyp glycosylation occurs in two distinct modes: Hyp arabinosylation and Hyp galactosylation. The Hyp contiguity hypothesis predicts arabinosylation of contiguous Hyp residues and galactosylation of clustered non-contiguous Hyp. Elucidation of Hyp glycosylation codes involves the design and expression of putative glycomotifs as simple repetitive peptides. Thus, repetitive (Ser-Hyp), directed Hyp galactosylation resulting in the exclusive addition of arabinogalactan polysaccharide to all the non-contiguous Hyp residues, and a new AGP. Another repetitive peptide from gum arabic glycoprotein, containing both contiguous and non-contiguous Hyp, directed both modes of Hyp glycosylation. Furthermore, expression of the (Ser-Hyp,), series confirmed the arabinosylation of contiguous Hyp. Thus. the Hyp contiguity hypothesis is a useful predictive tool in the functional genomics toolbox. (C) 2001 Elsevier Science Ltd. All rights reserved.