The use of dinoflagellate bioluminescence to characterize cell stimulation in bioreactors
The use of dinoflagellate bioluminescence to characterize cell stimulation in bioreactors
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DOI：
10.1002/bit.10647
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发表时间：
2003-07-05
影响因子：
3.8
通讯作者：
Frangos, JA
中科院分区：
文献类型：
--
作者：
Chen, AK;Latz, MI;Frangos, JA
生物发光甲藻是一种对流动敏感的海洋生物，在流体剪切的刺激下几乎瞬间产生光发射，并以剪切应力剂量依赖的方式。在目前的研究中，我们验证了这样一个假设，即通过悬浮甲藻监测生物发光可以作为一种工具来表征搅拌生物反应器中细胞对水动力的响应。进行了具体的研究，以确定：(1)具有最小细胞激活的叶轮结构，(2)细胞响应与综合剪切系数的相关性，以及(3)在搅拌中快速加速的效果。结果表明：(1)在体积传质系数为3×10~(-4)·S(~(-1))时，船用叶轮构型的刺激性小于拉什顿构型；(2)生物发光响应与修正的体积积分剪切因子具有很好的相关性；(3)搅拌中的快速加速具有很强的刺激性，表明剪切的时间梯度对增加细胞刺激有深远的影响。通过使用生物发光刺激作为微载体培养中搅拌诱导的细胞刺激和/或损伤的指示器，本研究允许验证假设和开发生物反应器中细胞损伤的新机制。(C)2003年威利期刊公司。
Bioluminescent dinoflagellates are flow-sensitive marine organisms that produce light emission almost instantaneously upon stimulation by fluid shear in a shear stress dose-dependent manner. In the present study we tested the hypothesis that monitoring bioluminescence by suspended dinoflagellates can be used as a tool to characterize cellular response to hydrodynamic forces in agitated bioreactors. Specific studies were performed to determine: (1) impeller configurations with minimum cell activation, (2) correlations of cellular response and an integrated shear factor, and (3) the effect of rapid acceleration in agitation. Results indicated that (1) at a volumetric mass transfer coefficient of 3 x 10(-4) s(-1), marine impeller configurations were less stimulatory than Rushton configurations, (2) bioluminescence response and a modified volumetric integrated shear factor had an excellent correlation, and (3) rapid acceleration in agitation was highly stimulatory, suggesting a profound effect of temporal gradients in shear in increasing cell stimulation. By using bioluminescence stimulation as an indicator of agitation-induced cell stimulation and/or damage in microcarrier cultures, the present study allows for the verification of hypotheses and development of novel mechanisms of cell damage in bioreactors. (C) 2003 Wiley Periodicals, Inc.