Synthesis and characterization of histidine-phosphorylated peptides
Synthesis and characterization of histidine-phosphorylated peptides
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DOI：
10.1002/pro.5560060704
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发表时间：
1997-07-01
期刊：
影响因子：
8
通讯作者：
Turck, CW
中科院分区：
文献类型：
--
作者：
Medzihradszky, KF;Phillipps, NJ;Turck, CW
蛋白质的翻译后磷酸化是许多细胞过程中的重要事件。尽管丝氨酸、苏氨酸和酪氨酸的磷酸酯已被广泛研究，但对于由磷酸基团修饰的其他氨基酸，仅有有限的信息可用。蛋白质中磷酸组氨酸残基的形成最初在原核生物中发现，但最近在真核细胞中也被发现。我们描述了含磷酸组氨酸的肽的合成和分析方法，这是研究这种翻译后修饰在细胞过程中的作用的先决条件。
Posttranslational phosphorylation of proteins is an important event in many cellular processes. Whereas phosphoesters of serine, threonine, and tyrosine have been studied extensively, only limited information is available for other amino acids modified by a phosphate group. The formation of phosphohistidine residues in proteins was discovered originally in prokaryotic organisms, but also has been found recently in eukaryotic cells. We describe methods for the synthesis and analysis of phosphohistidine-containing peptides, a prerequisite for the investigation of the role of this posttranslational modification in cellular processes.