Characterization of a viroid-derived RNA promoter for the DNA-dependent RNA polymerase from Escherichia coli
Characterization of a viroid-derived RNA promoter for the DNA-dependent RNA polymerase from Escherichia coli
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DOI：
10.1021/bi025595k
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发表时间：
2002-05-21
期刊：
影响因子：
2.9
通讯作者：
Perreault, JP
中科院分区：
文献类型：
--
作者：
Pelchat, M;Grenier, C;Perreault, JP
本文将桃潜伏花叶类病毒（PLMVd）RNA中的自互补、自切割锤头状RNA序列赋予一种新的功能，即转录启动子功能。这种RNA启动子，它采用了发夹结构，可以利用大肠杆菌RNA聚合酶（RNAP）在体外转录，触发RNAP驱动的转录，并负责合成的特定起始的功能进行了描述。起始的基本条件是在环附近有一个碱基配对的尿苷。由至少六个核苷酸连接到相对不稳定的茎组成的环的存在显著增加了起始水平。最后，我们提出了几个见解的RNAP的机制，揭示了它的行为与RNA模板相比，DNA的不同。
This paper attributes a novel function, namely, that of transcriptional promoter, to the self-complementary, self-cleaving hammerhead RNA sequences found in RNA derived from the peach latent mosaic viroid (PLMVd). The features of this RNA promoter, which adopts a hairpin structure that can be utilized by Escherichia coli RNA polymerase (RNAP) for in vitro transcription, that trigger the RNAP driven transcription and are responsible for the specific initiation of synthesis are described. The essential requirement for initiation is a basepaired uridine adjacent to the loop. The presence of a loop composed of at least six nucleotides connected to a relatively unstable stem significantly increases the level of initiation. Finally, we present several insights into the mechanism of the RNAP which reveal that it behaves differently with an RNA template as compared to a DNA one.