[The relation between polymorphisms of apolipoprotein B gene and atherosclerotic cerebral infarction].
[The relation between polymorphisms of apolipoprotein B gene and atherosclerotic cerebral infarction].
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DOI：
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发表时间：
1999-08
影响因子：
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通讯作者：
L. Wang;Y. Gu;G. Wu
中科院分区：
文献类型：
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作者：
L. Wang;Y. Gu;G. Wu
目的探讨中国汉族人群载脂蛋白B（ApoB）基因XbaI位点变异与动脉粥样硬化性脑梗死（ACI）的相关性。方法采用聚合酶链式反应（PCR）技术，对150例ACI患者和301例北京汉族健康年龄、性别匹配个体的ApoB基因XbaI位点限制性片段长度多态性（RFLP）进行研究。结果在ACI组和对照组中，X-X-基因型是最常见的基因型（频率：0.907，0.948），我们没有发现X+X+基因型。两组的基因型分布处于Hardy-Weiberg平衡状态。中国汉族人中稀有等位基因 X+ 的频率显着低于白种人种（0.027 vs 0.418、0.454、0.479，P < 0.01）。与对照组相比，ACI 组罕见等位基因 X+ 的出现频率较高（0.053 vs 0.027，P < 0.05）。 ACI组TC水平明显升高（5.3±1.3）、（4.9±1.3）mmol/L（P < 0.05），Apo-AI、Apo-AI/ApoB水平较低（1.02±0.34）、（1.26±0.40）g/L（P < 0.01）；与ACI组中X-X-基因型水平相比，X+X-基因型与ApoB水平较高相关（0.89±0.29）g/L、（0.78±0.17）g/L（P < 0.05）。结论 ApoB基因X+等位基因可能与中国人群ACI有一定的相关性，可能是通过影响ApoB代谢而增加ACI的易感性。
OBJECTIVE To explore the association of variance at XbaI site of apolipoprotein B (ApoB) gene and atherosclerotic cerebral infarction (ACI) in Chinese Han population. METHODS Using polymerase chain reaction (PCR) techniques, we studied the restrict fragment length polymorphism (RFLP) at XbaI site of ApoB gene in 150 patients with ACI and 301 healthy age, sex-matched individuals from a population of Chinese Han nationality in Beijing. RESULTS In both ACI group and control group, X-X- genotype was the most frequent one (frequency: 0.907, 0.948) and we did not find X+X+ genotype. The distribution of genotypes in the two groups was at the Hardy-Weiberg equilibriums. The frequency of rare allele X+ was significantly lower in Chinese Han than that reported in Caucasians (0.027 vs 0.418, 0.454, 0.479, P < 0.01). The higher frequency of rare allele X+ was found in the ACI group as compared with the control group (0.053 vs 0.027, P < 0.05). markedly increased levels of TC (5.3 +/- 1.3), (4.9 +/- 1.3) mmol/L (P < 0.05), and low levels of Apo-AI, Apo-AI/ApoB in the ACI group were observed (1.02 +/- 0.34), (1.26 +/- 0.40) g/L, (P < 0.01); X+X- genotype was associated with higher levels of ApoB compared with the levels of X-X- genotype in the ACI group (0.89 +/- 0.29) g/L, (0.78 +/- 0.17) g/L (P < 0.05). CONCLUSION X+ allele of ApoB gene may be associated with ACI to some extent in the Chinese population, and presumbly through its effect on ApoB metabolism it increases the susceptibility to ACI.