The beta-core fragment of human chorionic gonadotrophin inhibits growth of Kaposi's sarcoma-derived cells and a new immortalized Kaposi's sarcoma cell line
The beta-core fragment of human chorionic gonadotrophin inhibits growth of Kaposi's sarcoma-derived cells and a new immortalized Kaposi's sarcoma cell line
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DOI：
10.1097/00002030-199706000-00003
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发表时间：
1997-05-01
期刊：
影响因子：
3.8
通讯作者：
Benelli, R
中科院分区：
文献类型：
--
作者：
Albini, A;Paglieri, I;Benelli, R
目的：卡波西肉瘤 (KS) 是一种通常与 HIV 感染相关的疾病，男性比女性更常见。可能涉及怀孕和性激素。据报道，尿液中的人绒毛膜促性腺激素 (hCG) 可抑制 KS 细胞系的生长，但不同制剂之间存在很大差异。尿液 hCG 通常含有游离形式的 hCG 亚基和游离 β 亚基片段（β 核心），可能具有生物活性。我们比较了 β 核心片段、β 亚基重组和尿 hCG 对 KS 永生细胞和梭形细胞的影响。 设计和方法：对新的永生 KS 细胞系进行表型和核型表征。测试了 hCG 及其纯化衍生物对该细胞系和原代 KS 梭形细胞生长的影响。使用吖啶橙/溴化乙锭染色证明了细胞凋亡的诱导。结果：β-核心片段在摩尔基础上具有最有效的生长抑制活性。用 β-核心处理 72 小时后，60-70% 的 KS 细胞显示凋亡细胞核。对内皮细胞没有观察到影响。结论：hCG的β-核心片段被证明是hCG分子中最有效的部分，诱导KS细胞的生长抑制和凋亡。因此，β-核心可能是治疗 KS 的最合适的 hCG 衍生物。
Objective: Kaposi's sarcoma (KS), a condition often associated with HIV infection, is more common in men than in women; pregnancy and sex hormones could be involved. Urinary human chorionic gonadrotrophin (hCG) has been reported to inhibit the growth of KS cell lines, with great variability among preparations. Urinary hCG often contains free forms of the hCG subunits and a fragment of the free beta-subunit, the beta-core, which may have biological activity. We compared the effect of the beta-core fragment, the beta-subunit recombinant and urinary hCG on KS immortal and spindle cells.Design and methods: A new immortal KS cell line was phenotypically and karyotypically characterized. The effects on growth of this cell line and of primary KS spindle cells by hCG and its purified derivatives were tested. induction of apoptosis was demonstrated using acridine orange/ethidium bromide staining.Results: The beta-core fragment harboured the most potent growth inhibitory activity on a molar basis. After 72 h of treatment with the beta-core, 60-70% of KS cells show apoptotic nuclei. No effects were observed on endothelial cells.Conclusions: The beta-core fragment of hCG proved to be the most effective part of the hCG molecule, inducing growth inhibition and apoptosis of KS cells. Thus, the beta-core could be the most appropriate hCG derivative for the therapy of KS.