Role of granulosa and theca cell interactions in ovarian follicular maturation
Role of granulosa and theca cell interactions in ovarian follicular maturation
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DOI：
10.1002/jemt.20304
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发表时间：
2006-06
影响因子：
2.5
通讯作者：
K. Tajima;M. Orisaka;H. Yata;K. Goto;K. Hosokawa;F. Kotsuji
中科院分区：
文献类型：
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作者：
K. Tajima;M. Orisaka;H. Yata;K. Goto;K. Hosokawa;F. Kotsuji
我们开发了一种培养系统，其中两种类型的卵巢卵泡细胞被允许附着到胶原膜的相对侧。使用这种体外细胞培养系统，我们研究了颗粒细胞和卵泡膜细胞相互作用对牛卵巢卵泡细胞形态、结构和功能的影响。在研究的第一部分，我们探讨了卵泡膜和颗粒细胞之间的相互作用如何影响细胞的形态和结构。本研究使用从早期发育阶段的牛卵泡收集的卵泡细胞进行。单独培养的颗粒细胞扁平，形成单层片。相比之下，与卵泡膜细胞一起培养的颗粒细胞是凸起的，并形成多层片。单独培养的卵泡膜细胞薄、平、呈梭形。与颗粒细胞一起培养的卵泡膜细胞也是梭形的;然而，与单独培养的卵泡膜细胞相比，它们看起来凸起并且更密集。在研究的第二部分中，研究了细胞相互作用在控制颗粒细胞和卵泡膜细胞的分化和生长中的可能作用。当卵泡发育的早期阶段分离卵泡细胞，卵泡膜细胞减少颗粒细胞的孕酮和卵泡素的生产和增强颗粒细胞的生长。相反，当从卵泡发育的晚期分离细胞时，卵泡膜细胞增加颗粒细胞的激素产生，并且不影响颗粒细胞的生长。卵泡膜细胞的生长和雄烯二酮的生产增加颗粒细胞的存在，无论卵泡细胞的起源。这些结果表明，两种类型的滤泡细胞之间的通信导致其形态，结构，生长和功能的相互调节。在卵泡成熟过程中，细胞间的相互作用似乎是控制卵泡细胞分化和生长的主要因素之一。与单独培养的颗粒细胞和卵泡膜细胞相比，共培养的细胞似乎具有与体内生长卵泡壁中的细胞更相似的形态和功能特征。因此，我们推测，这两种类型的卵泡细胞之间的相互作用是必不可少的原始结构和功能的牛卵泡壁的维持。Microsc. Res. Tech.，2006.© 2006 Wiley利斯公司
We developed a culture system in which two types of ovarian follicular cells were allowed to attach to opposite sides of a collagen membrane. Using this in vitro cell culture system, we studied the effects of granulosa‐ and theca‐cell interaction on the morphology, structure, and function of bovine ovarian follicular cells. In the first part of the study, we explored how the interaction between theca and granulosa cells affects the morphology and structure of the cells. This study was done using follicular cells collected from bovine ovarian follicles at the early developmental stage. Granulosa cells cultured alone were flattened, and formed a monolayer sheet. By contrast, granulosa cells cultured with theca cells were convex, and formed multilayer sheets. Theca cells cultured alone were thin, flat, and spindle‐shaped. Theca cells cultured with granulosa cells were also spindle‐shaped; however, they appeared convex and more densely packed when compared with theca cells cultured alone. In the second part of the study, the possible role of the cellular interaction in the control of differentiation and growth of granulosa and theca cells was investigated. When follicular cells were isolated from the early stage of follicular development, theca cells reduced progesterone and inhibin production by granulosa cells and augmented the growth of granulosa cells. When the cells were isolated from the late stage of follicular development, by contrast, theca cells augmented hormonal production by granulosa cells, and did not affect the growth of granulosa cells. The growth and androstenedione production by theca cells were increased by the presence of granulosa cells, irrespective of the origin of follicular cells. These results demonstrated that communication between two types of follicular cells results in reciprocal modulation of their morphology, structure, growth, and function. Cellular interactions seem to be one of the major factors controlling the differentiation and growth of the follicular cells during the follicular maturation process. In contrast to granulosa and theca cells cultured alone, cells in the coculture seemed to possess morphological and functional characteristics more similar to those of cells in the growing follicular wall in vivo. Thus, we speculate that the interaction between these two types of follicular cells is essential for the maintenance of original structure and function of the bovine follicular wall. Microsc. Res. Tech., 2006. © 2006 Wiley‐Liss, Inc.