Specificity of a target cell-derived stop signal for afferent axonal growth.
Specificity of a target cell-derived stop signal for afferent axonal growth.
复制标题
靶细胞衍生的传入轴突生长停止信号的特异性。
DOI：
10.1002/neu.480230511
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Mason,CA
中科院分区：
文献类型：
--
作者：
Baird,DH;Baptista,CA;Wang,LC;Mason,CA
通过一种新的模型培养系统，其中传入神经与纯化的靶神经元群体共培养，我们已经证明了中枢神经系统（CNS）内的靶细胞，小脑颗粒神经元，为其适当的传入神经，苔藓纤维提供“停止生长信号”。为了研究这种停止信号是否是传入特异性的，我们将颗粒神经元与其他小脑传入系统，即来自下橄榄核的攀爬纤维（通常与浦肯野神经元接触）和视网膜神经节细胞传入神经元（从不进入小脑）共培养。颗粒神经元不对这些传入神经中的任何一个发出停止信号。相反，脑桥苔藓传入层粘连蛋白生长良好，颗粒神经元上的生长减少，橄榄和视网膜纤维显示类似的增长层粘连蛋白单独或颗粒神经元上。此外，每个传入纤维在层粘连蛋白上显示出不同程度的成束和生长锥形态。因此，颗粒神经元发出的生长停滞信号被其适当的传入神经特异性地识别。此外，这三种类型的传入表现出不同的生长模式，在相同的非细胞和细胞基板，暗示不同类别的神经元，这将有助于特异性的突触形成的生长调节的不同分子特征。John Wiley & Sons，Inc.
With a novel model culture system in which afferents are co‐cultured with purified populations of target neurons, we have demonstrated that a target cell within the central nervous system (CNS), the cerebellar granule neuron, poses a „stop‐growing signal”︁ for its appropriate afferents, the mossy fibers. To ask whether this stop signal is afferent specific, we co‐cultured granule neurons with an other cerebellar afferent system, the climbing fibers from the inferior olivary nuclei, which normally contact Purkinje neurons, and with retinal ganglion cell afferents, which never enter the cerebellum. Granule neurons do not pose a stop signal to either of these afferents. In contrast to pontine mossy afferents that grow well on laminin and showed reduced outgrowth on granule neurons, both olivary and retinal fibers displayed similar growth on laminin alone or on granule neurons. In addition, each afferent showed different degrees of fasciculation and growth cone morphology on laminin. Thus, the growth arrest signal sent by granule neurons is specifically recognized by their appropriate afferents. Moreover, these three types of afferents exhibit varying growth patterns on the same noncellular and cellular substrates, implicating distinct molecular characteristics of growth regulation for different classes of neurons that would contribute to specificity of synapse formation. © 1992 John Wiley & Sons, Inc.