Metabolism of cytoplasmic triacylglycerol in cultured aortic smooth muscle cells.
Metabolism of cytoplasmic triacylglycerol in cultured aortic smooth muscle cells.
复制标题
培养的主动脉平滑肌细胞中细胞质三酰甘油的代谢。
DOI：
10.1139/o81-092
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Y. Stein
中科院分区：
文献类型：
--
作者：
O. Stein;G. Coetzee;Y. Stein
A turnover of cytoplasmic triacylglycerol was studied in cultured rat, rabbit, and bovine aortic smooth muscle cells. Cytoplasmic triacylglycerol was labeled with [3H]glycerol in the presence of oleic acid in the medium and its loss from the cell was studied in the presence of carrier glycerol. Multiple additions of Isuprel or dibutyryl cyclic AMP during the chase period did not enhance the loss of labeled triacylglycerol. The rate of hydrolysis of cellular triacylglycerol was unchanged in the absence or in the presence of 100 microM chloroquine. Modulation of cellular cholesterol content by addition of low density lipoprotein or high density apolipoprotein--sphingomyelin liposomes did not affect the residence time of the cellular triacylglycerol. We conclude that cytoplasmic triacylglycerol in cultured aortic smooth muscle cells is metabolized by an extralysosomal enzyme which is neither catecholamine responsive nor affected by modulation of cellular cholesterol.
