Cell-Free microRNA-214 From Urine as a Biomarker for Non-Muscle-Invasive Bladder Cancer.
Cell-Free microRNA-214 From Urine as a Biomarker for Non-Muscle-Invasive Bladder Cancer.
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尿液中无细胞的microRNA-214作为非肌肉侵入性膀胱癌的生物标志物。
DOI：
10.4111/kju.2013.54.11.791
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发表时间：
2013-11
期刊：
影响因子：
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通讯作者：
Kim WJ
中科院分区：
文献类型：
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作者：
Kim SM;Kang HW;Kim WT;Kim YJ;Yun SJ;Lee SC;Kim WJ
microRNA是小的非编码RNA，microRNA-214（miR-214）与抑制癌细胞生长、迁移和侵袭有关。本研究的目的是研究从尿液中分离的无细胞miR-214是否可用作非肌肉浸润性膀胱癌（NMIBC）的生物标志物。本研究共纳入138例原发性NMIBC患者和144例健康正常对照。采用定量聚合酶链反应（PCR）检测尿中游离miR-214水平，并比较NMIBC患者与对照组的临床病理参数。NMIBC患者的尿中无细胞miR-214水平显著高于对照组（20.08±3.21 vs. 18.96±2.68，p=0.002）。然而，对于NMIBC患者，无细胞miR-214的尿水平既没有分级也没有分期（p>0.05，每个）。当我们根据临床结果比较尿液中无细胞miR-214的水平时，复发患者的miR-214水平低于无复发患者（19.24±2.67 vs. 20.41±3.41，p=0.023）。相比之下，显示进展的NMIBC患者和未显示进展的NMIBC患者之间无细胞miR-214的尿液水平没有显著差异（p=0.919）。多变量考克斯回归分析显示，尿中无细胞miR-214水平是NMIBC复发的独立预测因子（风险比，2.011; 95%置信区间，1.027至3.937; p=0.041）。尿中无细胞miR-214水平可能是NMIBC复发的独立预后参数。因此，尿无细胞microRNA-214可能是NMI BC的有用预后标志物。
MicroRNAs are small noncoding RNAs and microRNA-214 (miR-214) has been associated with the inhibition of cancer cell growth, migration, and invasion. The aim of this study was to investigate whether cell-free miR-214 isolated from urine could be used as a biomarker for non-muscle-invasive bladder cancer (NMIBC). A total of 138 patients with primary NMIBC and 144 healthy normal controls were enrolled in this study. By use of quantitative polymerase chain reaction (PCR), the urinary levels of cell-free miR-214 were measured and the clinicopathological parameters of patients with NMIBC were compared with those of the controls. The urinary levels of cell-free miR-214 were significantly higher in the NMIBC patients than in the controls (20.08±3.21 vs. 18.96±2.68, p=0.002). However, the urinary levels of cell-free miR-214 were neither graded nor staged for the NMIBC patients (p>0.05, each). When we compared the urinary levels of cell-free miR-214 according to clinical outcomes, patients with recurrence had lower levels of miR-214 than did those with no recurrence (19.24±2.67 vs. 20.41±3.41, p=0.023). By contrast, there were no significant differences in the urinary level of cell-free miR-214 between the NMIBC patients showing progression and those showing no progression (p=0.919). Multivariate Cox regression analysis revealed that urinary levels of cell-free miR-214 were an independent predictor of NMIBC recurrence (hazard ratio, 2.011; 95% confidence interval, 1.027 to 3.937; p=0.041). Urinary levels of cell-free miR-214 could be an independent prognostic parameter for NMIBC recurrence. Thus, urinary cell-free microRNA-214 might be a useful prognostic marker for NMI BC.