Multifunctional phosphate-based glass fibres prepared via electrospinning of coacervate precursors: controlled delivery, biocompatibility and antibacterial activity
Multifunctional phosphate-based glass fibres prepared via electrospinning of coacervate precursors: controlled delivery, biocompatibility and antibacterial activity
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DOI：
10.1016/j.mtla.2020.100939
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发表时间：
2020-12-01
期刊：
影响因子：
3.4
通讯作者：
Carta, Daniela
中科院分区：
文献类型：
--
作者：
Foroutan, Farzad;Nikolaou, Athanasios;Carta, Daniela
人们对既能迅速促进愈合又能预防感染的新型生物材料的需求越来越大。由于磷酸盐玻璃纤维是可生物吸收的，它可以同时诱导组织再生并以受控的方式输送治疗剂(例如抗菌离子)。在这里，我们提出了一系列的铜掺杂磷酸盐玻璃纤维在P_2O_5-CaO-Na_2O-(CuO)(X)(x=0，1，3和5摩尔%)系统中首次通过电纺凝聚体前驱体制备。与传统的高温熔融纺丝相比，这种方法有几个优点；凝聚体的合成是在室温和水溶液中进行的，允许加入温度敏感的分子。此外，静电纺丝是一种廉价、可持续、易于扩展的制造工艺。所生产的纤维呈棉状，完全无定形，平均直径在1-3微米之间。阳离子(Ca2+、Na+和Cu2+)和磷酸阴离子(PO43-、P2O74-和P3O105-)的溶解研究表明，随着时间的推移，所有物种在溶液中逐渐释放，非常适合受控药物输送应用。研究发现，对革兰氏阳性菌(金黄色葡萄球菌)和革兰氏阴性菌(大肠杆菌)的抗菌活性都随着Cu2+含量的增加而增加，并且对大肠杆菌的抗菌活性比对金黄色葡萄球菌更有效。有趣的是，Cu2+的含量似乎对细胞相容性有积极的影响。对成骨细胞样的MG63骨肉瘤细胞进行的测试表明，掺铜磷酸盐玻璃纤维再生骨组织的能力明显好于未掺杂的玻璃纤维。这些发现表明，磷酸盐基玻璃纤维在抗菌活性和组织再生方面是很有前途的多功能生物材料。
There is an increasing demand for new biomaterials that both rapidly stimulate healing and prevent infections. Being bioresorbable, phosphate-glass fibres can simultaneously induce tissue regeneration and deliver therapeutic agents (e.g. antibacterial ions) in a controlled way. Here we present a series of copper-doped phosphate-based glass fibres in the P2O5-CaO-Na2O-(CuO)(x) system (x = 0, 1, 3, and 5 mol%) prepared for the first time via electrospinning of coacervate precursors. This method presents several advantages over the conventional high temperature melt spinning; the synthesis of the coacervate occurs at room temperature and in aqueous solution allowing the incorporation of temperature sensitive molecules. Moreover, electrospinning is an inexpensive, sustainable, easily scalable manufacturing process. The fibres produced are cotton-like, fully amorphous with an average diameter in the range 1-3 mu m. Dissolution studies on cations (Ca2+, Na+ and Cu2+) and phosphate anions (PO43-, P2O74-, and P3O105-) show a gradual release of all species in solution over time, ideal for controlled drug delivery applications.The antibacterial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria was found to increase with increasing Cu2+ content and was found to be more effective against E. coli than against S. aureus. Interestingly, Cu2+ content seem to have a positive effect on cytocompatibility. Tests performed on osteoblast-like MG63 osteosarcoma cells have shown that the copper doped phosphate-glass fibres have a significantly better capacity to regenerate bone tissue than the undoped glass fibres. These findings suggest that phosphate-based glass fibres are promising multifunctional biomaterials for both antibacterial activity and tissue regeneration.