Gas-phase basicities of deprotonated matrix-assisted laser desorption/ionization matrix molecules
Gas-phase basicities of deprotonated matrix-assisted laser desorption/ionization matrix molecules
复制标题
DOI：
10.1016/s1387-3806(98)14200-8
复制
发表时间：
1999-03-15
影响因子：
1.8
通讯作者：
Zenobi, R
中科院分区：
文献类型：
--
作者：
Breuker, K;Knochenmuss, R;Zenobi, R
采用改进的包套法测定了8种基质辅助激光解吸/电离（MALDI）基质阴离子的气相碱度（GB）。参考阴离子通过激光解吸从新开发的两相液体/固体混合基质中产生，允许在明确的温度（299 K）和低压条件下进行测量。碱性最强的是3-氨基喹啉阴离子（GB = 1451 kJ/mol），碱性最低的是2,4,6-三羟基苯乙酮阴离子（GB = 1324 kJ/mol）。MALDI过程的后果讨论了有关基质离子形成，寡核苷酸和蛋白质分析。基态质子转移羽流反应在基质阴离子和分析物分子之间被发现在许多情况下能量有利。[J] .质谱分析与分析[J] . 1999 (2): 1 -3
The gas-phase basicities (GB) of eight matrix-assisted laser desorption/ionization (MALDI) matrix anions were determined using a modified bracketing method. Reference anions were generated by laser desorption from a newly developed two-phase liquid/solid mixed matrix, allowing measurements at a well defined temperature (299 K) and under low pressure conditions. The most basic matrix anion found was that of 3-aminoquinoline (GB = 1451 kJ/mol) and the least basic was the 2,4,6-trihydroxyacetophenone anion (GB = 1324 kJ/mol). Consequences for the MALDI process are discussed with respect to matrix ion formation, oligonucleotide and protein analysis. Ground-state proton transfer plume reactions between matrix anions and analyte molecules were found to be energetically favorable in many cases. (Int J Mass Spectrom 184 (1999) 25-38) (C) 1999 Elsevier Science B.V.