Which is the urine sample material of choice for metabolomics-driven biomarker studies?
Which is the urine sample material of choice for metabolomics-driven biomarker studies?
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代谢组学驱动的生物标志物研究选择哪种尿液样本材料？
DOI：
10.1016/j.aca.2020.01.028
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发表时间：
2020-04-08
影响因子：
6.2
通讯作者：
Xu, Guowang
中科院分区：
文献类型：
--
作者：
Liu, Xinyu;Yin, Peiyuan;Xu, Guowang
基于尿液代谢组学驱动的生物标志物发现策略在分析化学中得到越来越多的开发与应用。然而，目前缺乏基于数据的关于首选尿液样本材料的有效建议。我们对晨尿和第二次晨尿（MU）进行了研究，这两种是最常用的尿液样本。我们探究了可能导致这些样本材料中代谢组学生物标志物结果出现偏差的主要因素。首先，在禁食一夜后，从健康年轻男性中收集了35份晨尿和第二次晨尿样本。随后，将这些人分为两个亚组，一组在午餐和晚餐食用快餐（n = 17），另一组食用素食（n = 18）。之后再次收集晨尿和第二次晨尿。采用非靶向液相色谱 - 质谱法进行分析。在5400多个尿液离子特征中，超过一半的特征在晨尿和第二次晨尿之间存在显著差异。前一天仅两顿快餐就显著影响了晨尿和第二次晨尿中约30% 的所有代谢物。相比之下，两顿素食对第二次晨尿的影响较小。此外，我们确定了尿液中的47种代谢物，这些可能是生物标志物研究中的重要发现，但它们易受样本采集前一天饮食的影响。在通过饮食控制研究进行验证之前，应对这些代谢物谨慎处理。基于我们的研究结果，我们认为第二次晨尿最适合用于尿液生物标志物的有效分析，理想情况是在前一天食用标准化素食且只饮水后采集。（C）2020爱思唯尔出版社。保留所有权利。
Urine-based metabolomics-driven strategies for the discovery of biomarkers are increasingly developed and applied in analytical chemistry. But valid, data-based recommendations for a urine sample material of choice are lacking. We investigated first and second morning urine (MU), which are the most commonly used urine specimens. Potential major factors biasing metabolomics biomarker results in these sample materials were studied. First, 35 1st and 2nd MU samples were collected from healthy, young men after an overnight fast. Subsequently, two subgroups were built, one having fast food at lunch and dinner (n = 17), the other vegetarian meals (n = 18). Again 1st and 2nd MU were collected. Nontargeted liquid chromatography-mass spectrometry was applied for analyses. More than half of the >5400 urinary ion features showed a significant difference between 1st and 2nd MU. Just two fast food meals on previous day significantly affected around 30% of all metabolites in 1st and 2nd MU. In contrast, the effects of two vegetarian meals in 2nd MU were only minor. Additionally, we describe 47 metabolites in urine, possible hits in biomarker studies, which are susceptible to the diet the day before sample collection. They should be handled with caution until validation in diet-controlled studies. Based on our results we think the second MU, ideally collected after standardized vegetarian meals and drinking only water on the previous day, is most suitable for valid analysis of biomarkers in urine. (C) 2020 Elsevier B.V. All rights reserved.