Biological activity of chicken calcitonin: effects on neonatal rat and embryonic chick osteoclasts.
Biological activity of chicken calcitonin: effects on neonatal rat and embryonic chick osteoclasts.
复制标题
鸡降钙素的生物活性：对新生大鼠和雏鸡破骨细胞的影响。
DOI：
10.1002/jbmr.5650020512
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Arnett,TR
中科院分区：
文献类型：
--
作者：
Dempster,DW;Murrills,RJ;Horbert,WR;Arnett,TR
Chicken calcitonin (cCT) has recently been synthesized according to nucleotide sequence data. We have investigated thein vitroeffects of this hormone on the activity of disaggregated, neonatal rat and embryonic chick osteoclasts. While synthetic cCT inhibited bone resorption by neonatal rat osteoclasts at concentrations as low as 0.1 pg/ml, it failed to produce a significant reduction in bone resorption by embryonic chick osteoclasts, even at concentrations as high as 1 μg/ml. Similarly, cCT at 1 pg/ml reproducibly produced the rapid and dramatic inhibition of rat osteoclast motility characteristic of other calcitonins, as judged by time‐lapse video recording, but did not impair the motile behavior of chick osteoclasts at concentrations up to a million‐fold higher. Previous studies showing that embryonic chick osteoclasts were unresponsive to synthetic salmon calcitonin left open the possibility that the native hormone was required to produce an inhibitory effect. We conclude that the osteoclast is not a target cell for calcitonin in the embryonic chick; further studies will be required to define a role, if any, for this potent but enigmatic hormone in birds.
