Identification and characterization of a new member of the TNF family that induces apoptosis
Identification and characterization of a new member of the TNF family that induces apoptosis
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DOI：
10.1016/1074-7613(95)90057-8
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发表时间：
1995-12-01
期刊：
影响因子：
32.4
通讯作者：
Goodwin, RG
中科院分区：
文献类型：
--
作者：
Wiley, SR;Schooley, K;Goodwin, RG
一种新的肿瘤坏死因子（TNF）家族成员已被克隆和鉴定。这种蛋白质，称为TNF-相关的凋亡诱导配体（TRAIL），由281和291氨基酸的人和鼠的形式，分别共享65%的氨基酸同一性。TRAIL是一种II型膜蛋白，其C-末端胞外结构域与其他TNF家族成员具有明显的同源性。TRAIL转录物在多种人体组织中检测到，最主要的是在脾、肺和前列腺中。TRAIL基因位于3号染色体上的3q 26位置，其不接近任何其他已知的TNF配体家族成员。全长细胞表面表达的TRAIL和皮摩尔浓度的可溶性TRAIL在各种不同来源的转化细胞系中迅速诱导凋亡。
A novel tumor necrosis factor (TNF) family member has been cloned and characterized. This protein, designated TNF-related apoptosis-inducing ligand (TRAIL), consists of 281 and 291 aa in the human and murine forms, respectively, which share 65% aa identity. TRAIL is a type II membrane protein, whose C-terminal extracellular domain shows clear homology to other TNF family members. TRAIL transcripts are detected in a variety of human tissues, most predominantly in spleen, lung, and prostate. The TRAIL gene is located on chromosome 3 at position 3q26, which is not close to any other known TNF ligand family members. Both full-length cell surface expressed TRAIL and picomolar concentrations of soluble TRAIL rapidly induce apoptosis in a wide variety of transformed cell lines of diverse origin.