Production of somatic hybrid plants between Iris ensata Thunb. and I. germanica
Production of somatic hybrid plants between Iris ensata Thunb. and I. germanica
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Iris ensata Thunb 体细胞杂交植物的生产。
DOI：
10.1023/a:1026431800693
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发表时间：
2004
期刊：
影响因子：
1.9
通讯作者：
T. Adachi
中科院分区：
文献类型：
--
作者：
K. Shimizu;Y. Miyabe;H. Nagaike;T. Yabuya;T. Adachi
通过传统有性杂交无法实现的广泛杂交现在可以通过体细胞杂交来实现。通过电融合将 I. ensata 和 I. germanica 的原生质体融合。为了选择体细胞杂种，将在原生质体培养中不形成集落的I.ensata原生质体和仅对白化芽具有再生能力的I.germanica原生质体用于对称融合。另一方面，通过碘乙酰胺(IOA)处理灭活的I.ensata和I.germanica原生质体用于不对称融合。细胞融合后5-6个月，对称和不对称融合获得绿色植物。在随机扩增多态性 DNA (RAPD) 分析中，绿色植物具有两个亲本物种特有的条带。因此，这些植物是 I. ensata 和 I. germanica 之间的体细胞杂种。
Wide hybridization that cannot be attained through conventional sexual crosses, can now be approached by somatic hybridization. Protoplasts of I. ensata and I. germanica were fused by electrofusion. For the selection of somatic hybrids, protoplasts of I. ensata which did not form colonies in protoplast culture and protoplasts of I. germanica which had regeneration ability for only albino shoot were used in symmetric fusion. On the other hand, the protoplasts of I. ensata and I. germanica protoplasts which were inactivated by iodoacetamide (IOA) treatment were used in asymmetric fusion. Five-six months after cell fusion, green plants were obtained in the symmetric and asymmetric fusion. In the random amplified polymorphic DNA (RAPD) analysis, the green plants had bands specific to both parental species. Therefore, these plants were somatic hybrids between I. ensata and I. germanica.