Tissue-specific TAFs counteract polycomb to turn on terminal differentiation
Tissue-specific TAFs counteract polycomb to turn on terminal differentiation
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DOI：
10.1126/science.1118101
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发表时间：
2005-11-04
期刊：
影响因子：
56.9
通讯作者：
Fuller, MT
中科院分区：
文献类型：
--
作者：
Chen, X;Hiller, M;Fuller, MT
Polycomb转录沉默机制与前体命运的维持有关，但这种抑制如何逆转以允许细胞分化尚不清楚。这里我们展示了睾丸特异性TAF。（TBP相关因子）同源物所需的终端分化的配偶生殖细胞可以激活靶基因的表达，部分抵消抑制Polycomb。染色质免疫沉淀显示，睾丸TAFs结合到目标启动子，减少Polycomb结合，并促进局部积累的H3K4me3，一个标志的Trithorax行动。睾丸TAFs还促进了Polycomb抑制复合物1组分在精母细胞核仁中的重新定位，暗示了终末分化调节中的亚核结构。
Polycomb transcriptional silencing machinery is implicated in the maintenance of precursor fates, but how this repression is reversed to allow cell differentiation is unknown. Here we show that testis-specific TAF. (TBP-associated factor) homologs required for terminal differentiation of mate germ cells may activate target gene expression in part by counteracting repression by Polycomb. Chromatin immunoprecipitation revealed that testis TAFs bind to target promoters, reduce Polycomb binding, and promote local accumulation of H3K4me3, a mark of Trithorax action. Testis TAFs also promoted relocalization of Polycomb Repression Complex 1 components to the nucleolus in spermatocytes, implicating subnuclear architecture in the regulation of terminal differentiation.