Identification of distinct myocardin splice variants in the bladder.
Identification of distinct myocardin splice variants in the bladder.
复制标题
膀胱中不同心肌蛋白剪接变体的鉴定。
DOI：
10.1016/j.juro.2009.03.079
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
McHugh,KirkM
中科院分区：
文献类型：
--
作者：
Saha,Monalee;Ingraham,SusanE;Carpenter,Ashley;Robinson,Melissa;McHugh,KelseyE;Singh,Sunita;Robinson,MichaelL;McHugh,KirkM
目的：尽管肌钙蛋白在平滑肌发育中的重要性已得到充分证实，但许多组织特异性的平滑肌分化的复杂机制仍有待确定。我们研究了肌钙蛋白在发育中的膀胱和成人膀胱中的表达，以确定可能在逼尿肌平滑肌发育中起作用的潜在的组织特异性差异。材料与方法利用逆转录酶和定量聚合酶链式反应来检测肌钙蛋白在小鼠和人膀胱中的表达，并与其他组织进行比较。结果对小鼠的肌钙蛋白基因进行了Exonic图谱分析，发现了一系列独特的肌钙蛋白剪接变异体，这些剪接变体来自于先前发现的肌钙蛋白基因的外显子2和3之间的一个新的305个核苷酸的外显子。每个变异体在小鼠中表现出不同的表达模式，初级蛋白质序列表明每个已识别的肌钙蛋白变异体具有独特的功能。在人类膀胱中也观察到了相同的肌钙蛋白剪接变体，以及在小鼠中没有观察到的人类特有的第12外显子肌钙蛋白剪接变体。结论鉴定出一系列在膀胱和其他肌肉和非肌肉组织中差异表达的独特的肌钙蛋白剪接变体，为调节与肌钙蛋白转录程序相关的许多独特的组织特异性功能提供了潜在的分子平台。
PurposeAlthough the importance of myocardin in smooth muscle development is well established, many tissue specific intricacies of smooth muscle differentiation remain to be determined. We characterized myocardin expression in the developing and adult bladder to identify potential tissue specific differences that may have a role in detrusor smooth muscle development.Materials and MethodsReverse transcriptase and quantitative polymerase chain reaction were done to determine myocardin expression in the mouse and human bladder vs various other tissues. Sequence analysis was done to confirm the genomic location of the various polymerase chain reaction products.ResultsExonic profiling of the mouse myocardin gene identified a series of unique myocardin splice variants derived from a novel 305 bp exon between exons 2 and 3 of the previously identified myocardin gene. Each variant showed a differential pattern of expression in the mouse and primary protein sequences suggested a unique function for each myocardin variant identified. Identical myocardin splice variants were also observed in the human bladder as well as a unique human specific exon 12 myocardin splice variant that was not observed in the mouse.ConclusionsIdentifying a series of unique myocardin splice variants that are differentially expressed in the bladder, and other muscle and nonmuscle tissues provides a potential molecular platform for mediating many unique tissue specific functions associated with the myocardin transcriptional program.
影响因子：
15.9
作者：
McDonald, OG;Wamhoff, BR;Owens, GK
通讯作者：
Owens, GK
鉴定出一种独特的转基因小鼠品系，该小鼠品系会出现巨膀胱、梗阻性尿路病和肾功能障碍。
DOI：
10.1681/asn.2006040405
发表时间：
2007
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
Singh,Sunita;Robinson,Melissa;Nahi,Fatin;Coley,Brian;Robinson,MichaelL;Bates,CarltonM;Kornacker,Karl;McHugh,KirkM
通讯作者：
McHugh,KirkM