Extracellular antimutagenic activities of selected probiotic Bifidobacterium and Lactobacillus spp. as a function of growth phase
Extracellular antimutagenic activities of selected probiotic Bifidobacterium and Lactobacillus spp. as a function of growth phase
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DOI：
10.1080/03601230701795262
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发表时间：
2008-02-01
影响因子：
2
通讯作者：
Ricke, S. C.
中科院分区：
文献类型：
--
作者：
Chalova, V. I.;Lingbeck, J. M.;Ricke, S. C.
从乳杆菌属和双歧杆菌属中筛选出的菌株产生对苯并[a]芘(BaP)和叠氮化钠(SA)具有抗突变作用的胞外生物活性化合物的能力随生长阶段的变化而变化。指数相和稳定期的细菌上清液对这两种诱变剂具有不同的抗突变活性。在指数生长过程中，所有乳酸菌对BaP均无抗突变作用或表现出较低的抗突变活性。在金黄色葡萄球菌的固定相中也观察到较高的抗突变活性或乳酸菌上清。在指数期表现出较低的诱变抑制率(pI=28.14+/-7.41)，在稳定期无抗突变活性。在双歧杆菌中，只有青春型双歧杆菌ATCC 15703对BaP表现出较高的指数期抗突变活性。然而，相同的细菌上清液在指数期和稳定期对SA都没有任何抗突变作用。双歧杆菌ATCC 11863在指数相和静止相对BaP或SA没有表现出明显的活性差异。只有B.breve ATCC 15700在稳定期对金黄色葡萄球菌表现出较强的抗诱变作用，而在指数生长期则没有表现出抗诱变作用。总体而言，细菌抗突变反应与生长阶段和诱变剂类型有关。
The capabilities of selected strains from genera Lactobacillus and Bifidobacterium to produce extracellular bioactive compounds with antimutagenic properties against benzo[a]pyrene (BaP) and sodium azide (SA) were tested as a function of growth phase. The bacterial supernatants from exponential and stationary phases were characterized with different patterns of antimutagenic activity against the two mutagens. All lactobacilli exhibited either no effect or low antimutagenicity against BaP during exponential growth. Higher antimutagenic activities or lactobacilli supernatants were observed in the stationary phase against SA as well. An exception was Lactobacillus sakei 23K which expressed a relatively low percent of inhibition of mutagenesis (PI = 28.14 +/- 7.41) in the exponential phase and no antimutagenic activity in the stationary phase. Of the bifidobacteria, only Bifidobacterium adoleascentis ATCC 15703 exhibited higher antimutagenecity against BaP in the exponential phase. The same bacterial supernatants however, did not possess any antimutagenicity against SA in either the exponential or stationary phases. B. bifidum ATCC 11863 did not express any significant differences in its activity against either BaP or SA in the exponential or stationary phases. Only B. breve ATCC 15700 expressed a high antimutagenic effect against SA in the stationary phase but exhibited no effect during exponential growth. Overall, bacterial antimutagenic responses were associated with growth phase and type of mutagen.