Chondroitin sulfate expression around motoneurons changes after complete spinal transection of neonatal rats
Chondroitin sulfate expression around motoneurons changes after complete spinal transection of neonatal rats
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DOI：
10.1016/j.neulet.2021.136324
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发表时间：
2021-11-14
影响因子：
2.5
通讯作者：
Funakoshi，Kengo
中科院分区：
文献类型：
--
作者：
Takiguchi，Masahito;Akaike，Takashi;Funakoshi，Kengo
新生大鼠脊髓完全横断（CST）后后肢运动活动可自发恢复，但其恢复机制尚不清楚。神经元胞体周围的神经元周网（PNN）包括细胞外基质，在发育过程中调节神经元的可塑性。在这里，我们研究了硫酸软骨素（CS）的表达，一个主要组成部分的PNN，运动神经元后CST的新生大鼠，并将其与幼年大鼠，其中后肢运动活动不能自发恢复。脊髓横断在胸中段水平的新生大鼠（出生后5天[P5]和P10）和幼年大鼠（P15和P20）。CST后两周，P10时CST大鼠中被硫酸软骨素C（CS-C）−阳性结构包围的运动神经元的百分比显著低于完整大鼠，P15时CST大鼠中被硫酸软骨素C（CS-C）−阳性结构包围的运动神经元的百分比倾向于高于完整大鼠。P15时CST大鼠PNN中CS-A −阳性结构的运动神经元百分比显著低于正常大鼠，提示CST大鼠PNN中CS-A和CS-C的表达存在差异。CS-C −阳性PNNs的减少可能促进与运动神经元形成新的突触接触，导致新生期CST大鼠后肢运动活动的恢复。
Hind limb locomotor activity spontaneously recovers after complete spinal transection (CST) in neonatal rats, but the mechanisms underlying the recovery are poorly understood. The perineuronal net (PNN) surrounding the neuronal cell bodies comprises an extracellular matrix that regulates neuronal plasticity during development. Here, we examined the expression of chondroitin sulfate (CS), a major component of the PNN, on motoneurons after CST in neonatal rats, and compared it with that in juvenile rats, in which hindlimb locomotor activity does not recover spontaneously.The spinal cord was transected at the mid-thoracic level in neonatal (postnatal day 5 [P5] and P10) and juvenile (P15 and P20) rats. Two weeks after CST, the percentage of motoneurons surrounded by chondroitin sulfate C (CS-C) − positive structures was significantly lower in rats with CST at P10 than in intact rats, and tended to be higher in rats with CST at P15 than in intact rats. The percentage of motoneurons with CS-A − positive structures was significantly lower in rats with CST at P15 than in intact rats.These findings suggest that CS-A and CS-C are differentially expressed in the PNNs in rats with CST. The decrease in CS-C − positive PNNs might facilitate the formation of new synaptic contacts to motoneurons, resulting in the recovery of the hindlimb locomotor activity in rats with CST during the neonatal period.