Versican V1 in human endometrial epithelial cells promotes BeWo spheroid adhesion in vitro
Versican V1 in human endometrial epithelial cells promotes BeWo spheroid adhesion in vitro
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DOI：
10.1530/rep-18-0333
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发表时间：
2019-01-01
期刊：
影响因子：
3.8
通讯作者：
Sato, Yukiyasu
中科院分区：
文献类型：
--
作者：
Miyazaki, Yumiko;Horie, Akihito;Sato, Yukiyasu
子宫内膜细胞外基质（ECM）是胚胎着床所必需的。Versican是一种结合透明质酸并形成大ECM聚集体的大硫酸软骨素蛋白聚糖，可影响基本生理现象，如细胞增殖、粘附和迁移。本研究探讨多功能蛋白聚糖在人类胚胎着床过程中的可能作用。Versican V1在人子宫内膜上皮细胞（EECs）中的表达和分泌在分泌中期最为突出。雌激素和孕激素处理后，EECs中Versican的表达显著增加，但单独雌激素处理后，Versican的表达没有增加。我们还建立了多功能蛋白聚糖V1过表达的石川（子宫内膜癌细胞系）细胞（ISKW-V1）、多功能蛋白聚糖V3过表达的（ISKW-V3）和对照GFP过表达的（ISKW-GFP）石川细胞。通过体外植入模型，发现BeWo（绒毛膜癌细胞系）球状体与ISKW-V1单层的附着率比与ISKW-GFP单层的附着率显著增强，但与ISKW-V3单层的附着率相比没有显著增强。来自ISKW-V1（V1-CM）的条件培养基也促进BeWo球状体与ISKW单层的附着。然而，当V1-CM用降解硫酸软骨素的软骨素酶ABC预处理时，这种附着促进作用被取消。因此，在ECM成分中，多功能蛋白聚糖V1可能有助于人类胚胎植入。
The endometrium extracellular matrix (ECM) is essential for embryo implantation. Versican, a large chondroitin sulfate proteoglycan that binds hyaluronan and forms large ECM aggregates, can influence fundamental physiological phenomena, such as cell proliferation, adhesion and migration. The present study investigated the possible role of versican in human embryo implantation. Versican V1 expression and secretion in human endometrial epithelial cells (EECs) was most prominent in the mid-secretory phase. Versican expression in EECs significantly increased after treatment with estrogen and progesterone, but not by estrogen alone. We also established versican V1-overexpressing Ishikawa (endometrial cancer cell line) cells (ISKW-V1), versican V3-overexpressing (ISKW-V3) and control GFP-overexpressing (ISKW-GFP) Ishikawa cells. By the in vitro implantation model, the attachment ratio of BeWo (choriocarcinoma cell line) spheroids to the monolayer of ISKW-V1, but not of ISKW-V3, was found significantly enhanced compared with attachment to the ISKW-GFP monolayer. The conditioned medium derived from ISKW-V1 (V1-CM) also promoted the attachment of BeWo spheroids to the ISKW monolayer. However, this attachment-promoting effect was abolished when V1-CM was pretreated with chondroitinase ABC, which degrades chondroitin sulfate. Therefore, out of the ECM components, versican V1 may facilitate human embryo implantation.