Effect of mutations in genes affecting homologous recombination on restriction enzyme-mediated and illegitimate recombination in Saccharomyces cerevisiae.
Effect of mutations in genes affecting homologous recombination on restriction enzyme-mediated and illegitimate recombination in Saccharomyces cerevisiae.
复制标题
影响酿酒酵母同源重组的基因突变对限制性酶介导和非法重组的影响。
DOI：
10.1128/mcb.14.7.4493-4500.1994
复制
发表时间：
1994
影响因子：
5.3
通讯作者：
Petes,TD
中科院分区：
文献类型：
--
作者：
Schiestl,RH;Zhu,J;Petes,TD
Restriction enzyme-mediated events (REM events; integration of transforming DNA catalyzed by in vivo action of a restriction enzyme) and illegitimate recombination events (IR events; integration of transforming DNA that shares no homology with the host genomic sequences) have been previously characterized inSaccharomyces cerevisiae.This study determines the effect of mutations in genes that are involved in homologous recombination and/or in the repair of double-stranded DNA breaks on these recombination events. Surprisingly, REM events are completely independent of the double-strand-break repair functions encoded by theRAD51, RAD52, andRAD57genes but require theRAD50gene product. IR events are under different genetic control than homologous integration events. In therad50mutant, homologous integration occurred at wild-type frequency, whereas the frequency of IR events was 20- to 100-fold reduced. Conversely, therad52mutant was grossly deficient in homologous integration (at least 1,000-fold reduced) but showed only a 2- to 8-fold reduction in IR frequency.
