Caprine (Goat) Collagen: A Potential Biomaterial for Skin Tissue Engineering
Caprine (Goat) Collagen: A Potential Biomaterial for Skin Tissue Engineering
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DOI：
10.1163/092050610x551943
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发表时间：
2012-01-01
影响因子：
3.6
通讯作者：
Maiti, Tapas K.
中科院分区：
文献类型：
--
作者：
Banerjee, Indranil;Mishra, Debasish;Maiti, Tapas K.
目前用于组织工程的胶原蛋白主要是牛或猪来源的。然而，海绵状脑病流行的风险限制了这些来源的胶原蛋白的使用。考虑到上述观点，我们探索了使用次大陆的家养山羊作为组织工程应用胶原蛋白的潜在来源的可能性。本文介绍了酸溶性山羊肌腱胶原（GTC）的分离、理化特性、生物相容性研究及在创伤愈合中的应用。采用SDS-PAGE、氨基酸组成分析、红外光谱和圆二色谱对1%乙酸提取的GTC进行了理化表征。结果表明，GTC由I型胶原组成。生物相容性研究表明，GTC增强细胞粘附，细胞周期进程和增殖。结合牵引力显微镜的免疫细胞化学分析进一步证实了GTC中的上级粘着斑复合物介导的细胞-基质相互作用。最后，在小鼠模型中的体内研究显示，GTC具有低免疫原性，并且显著增强愈合过程。在整个研究过程中，将小牛皮肤胶原蛋白（CSC）用作比较评价的标准品。因此，GTC有可能作为皮肤组织工程的生物材料应用。(C)Koninklijke Brill NV，莱顿，2012
Collagens presently used in tissue engineering are primarily of bovine or porcine origin. However, a risk of a spongiform encephalopathy epidemic has limited the use of collagen from these sources. Keeping the aforementioned perspective in mind, we explored the possibility of using domestic goat available in the subcontinent as a potential source of collagen for tissue-engineering application. This article delineates the isolation, physico-chemical characterization, biocompatibility study and wound healing application of acid soluble caprine (goat) tendon collagen (GTC). Physico-chemical characterization of 1% acetic acid extracted GTC was done by SDS-PAGE, amino-acid composition analysis, FT-IR and CD spectroscopy. Results revealed that GTC was comprised of type-I collagen. Biocompatibility study showed that GTC augmented cell adhesion, cell cycle progression and proliferation. Immuno-cytochemical analysis in conjugation with traction force microscopy further confirmed a superior focal adhesion complex mediated cell-substrate interaction in GTC. Finally, in vivo study in mice model revealed that GTC has low immunogenicity and it augments healing process significantly. Throughout the study, calf skin collagen (CSC) was used as standard for comparative evaluation. In conclusion, it can be said that GTC may find its application as biomaterial in skin tissue engineering. (C) Koninklijke Brill NV, Leiden, 2012