Normal and tumor-derived organoids as a drug screening platform for tumor-specific drug vulnerabilities.
Normal and tumor-derived organoids as a drug screening platform for tumor-specific drug vulnerabilities.
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DOI：
10.1016/j.xpro.2021.101079
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Drost J
中科院分区：
文献类型：
--
作者：
Calandrini C;Drost J
患者来源的肿瘤类器官可以预测患者的治疗反应，而正常组织来源的类器官可以进行药物毒性测试。因此，结合两种类型的类器官可以筛选肿瘤特异性药物的脆弱性。在这里，我们提供了一个详细的协议，用于类器官药物筛选，作为原理证明，患者来源的恶性横纹肌样肿瘤类器官。该方案可适用于任何肿瘤和/或正常组织来源的类器官培养物的药物测试。有关本方案使用和执行的完整详细信息，请参阅。患者来源的类器官药物筛选方案适用于正常和肿瘤来源的类器官允许识别肿瘤特异性药物脆弱性患者来源的肿瘤类器官可以预测患者的治疗反应;正常组织来源的类器官允许药物毒性测试。结合这两种类型的类器官可以筛查肿瘤特异性药物的脆弱性。在这里，我们提供了一个详细的协议与患者源性恶性横纹肌样肿瘤的类器官药物筛选。该方案可适用于任何肿瘤和/或正常组织来源的类器官培养物的高通量药物测试。
Patient-derived tumor organoids can be predictive of patient’s treatment responses, and normal tissue-derived organoids allow for drug toxicity testing. Combining both types of organoids therefore enables screening for tumor-specific drug vulnerabilities. Here, we provide a detailed protocol for organoid drug screening using, as proof-of-principle, patient-derived malignant rhabdoid tumor organoids. The protocol can be adapted for drug testing on any tumor and/or normal tissue-derived organoid culture. For complete details on the use and execution of this protocol, please refer to. Protocol for patient-derived organoid drug screening Applicable to both normal and tumor-derived organoids Allows for the identification of tumor-specific drug vulnerabilities Patient-derived tumor organoids can be predictive of a patient’s treatment response; normal tissue-derived organoids allow for drug toxicity testing. Combining both types of organoids enables screening for tumor-specific drug vulnerabilities. Here, we provide a detailed protocol for organoid drug screening with patient-derived malignant rhabdoid tumors. The protocol can be adapted for high-throughput drug testing on any tumor and/or normal tissue-derived organoid culture.
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影响因子：
16.6
作者：
Calandrini, Camilla;Schutgens, Frans;Drost, Jarno
通讯作者：
Drost, Jarno
影响因子：
8.8
作者：
Calandrini, Camilla;van Hooff, Sander R.;Drost, Jarno
通讯作者：
Drost, Jarno
影响因子：
16.6
作者：
Kim, Minsuh;Mun, Hyemin;Jang, Se Jin
通讯作者：
Jang, Se Jin
影响因子：
7.5
作者：
Sigauke, E;Rakheja, D;Raisanen, J
通讯作者：
Raisanen, J
影响因子：
64.8
作者：
Drost, Jarno;van Jaarsveld, Richard H.;Clevers, Hans
通讯作者：
Clevers, Hans