Nanoscale optomechanical actuators for controlling mechanotransduction in living cells.
Nanoscale optomechanical actuators for controlling mechanotransduction in living cells.
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DOI：
10.1038/nmeth.3689
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发表时间：
2016-02
期刊：
影响因子：
48
通讯作者：
Salaita K
中科院分区：
文献类型：
--
作者：
Liu Z;Liu Y;Chang Y;Seyf HR;Henry A;Mattheyses AL;Yehl K;Zhang Y;Huang Z;Salaita K
在此，我们开发了一种光学控制受体张力的方法。这是通过使用非侵入性近红外光控制的光机械致动器纳米颗粒来实现的。照明导致粒子塌陷，以高空间和时间分辨率将皮牛顿力传递到特定的细胞表面受体。作为概念验证，我们应用光机械致动来触发基于整合素的粘着斑形成、细胞突起和迁移以及T细胞受体活化。
Herein we develop an approach for optically controlling receptor tension. This is achieved using optomechanical actuator nanoparticles that are controlled with non-invasive near-infrared light. Illumination leads to particle collapse, delivering piconewton forces to specific cell surface receptors with high spatial and temporal resolution. As a proof-of-concept, we applied optomechanical actuation to trigger integrin-based focal adhesion formation, cell protrusion and migration, as well as T cell receptor activation.